基于响应面和G1-熵权法优选彝药痛风颗粒提取工艺及其药效评价

目的 优选彝药痛风颗粒（Yi Medicine Tongfeng Granules,YMTG）的提取工艺，并探索其对痛风性肾病的作用，为其新药的开发与利用提供科学依据。方法 采用主客观组合赋权和响应面法设计，以加水倍数、浸泡时间、提取时间、提取次数为考察因素，新绿原酸、绿原酸、隐绿原酸、咖啡酸、虎杖苷、新落新妇苷、落新妇苷、异落新妇苷8种指标成分提取率及出膏率为评价指标，优选YMTG的最佳水提工艺，并以痛风性肾病模型进行药效评价。结果 优选YMTG最佳提取工艺为加12倍水、浸泡60 min、煎煮3次，每次煎煮80 min；药效结果表明，YMTG可降低痛风性肾病模型大鼠血液中尿酸质量浓度、增加尿酸排出量和尿酸清除率，降低血液中血清肌酐、尿素氮水平，减轻肾脏损伤。结论 优选的YMTG提取工艺稳定可行，其对痛风性肾病有良好的作用。

Optimization extraction process of Yi Medicine Tongfeng Granules based on response surface methodology and G1-entropy weight method and its pharmacodynamic evaluation

Objective To provide a scientific basis for the development and utilization of Yi Medicine Tongfeng Granules (YMTG) by optimizing the extraction process of YMTG and exploring its effect on gouty nephropathy. Methods In this study, multi-objective optimization of water extraction technology of YMTG was performed using a combination of the subjective-objective combined weighting method and the response surface method. The process parameters were considered as extraction duration, extraction times, immersion time, and water addition. The contents of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, polydatin, neoastilbin, astilbin, isoastilbin and the extract rate were selected as the objective functions. And gouty nephropathy model was used to evaluate the pharmacodynamics of YMTG. Results The results showed that the optimum process of decocting YMTG was as follow: soaking in 12 times water for 60 min, boiling three times for 8 min each time. The pharmacodynamics results showed that YMTG could reduce the concentration of uric acid in the blood of rats with gouty nephropathy, increase the excretion of uric acid and the clearance rate of uric acid, reduce the levels of serum creatinine and blood urea nitrogen, and alleviate renal injury. Conclusion The optimized extraction process of YMTG is stable and feasible, and which has a good effect on gouty nephropathy.