首页 | 本学科首页   官方微博 | 高级检索  
     

基因分型技术首次应用于类孟买型家系ABO基因的研究
引用本文:喻琼,吴国光,邓志辉,梁延连,苏宇清,赵桐茂. 基因分型技术首次应用于类孟买型家系ABO基因的研究[J]. 罕少疾病杂志, 2003, 10(2): 5-7
作者姓名:喻琼  吴国光  邓志辉  梁延连  苏宇清  赵桐茂
作者单位:1. 深圳市输血医学研究所,广东,深圳,518035
2. 美国国立卫生研究所 Bethesda USA
基金项目:广东省医学科研课题基金资助项目(2001641)
摘    要:目的 探计将基因分型技术用于解决临床输注中血型血清学难题。方法 报告首次在国内用基因分型技术用于一个类孟买型家系5口人ABO基因多态性的研究。在吸收放散试验、唾液血型物质凝集抑制等血清学试验基础上,采用快速盐析法提取外周血中的DNA,用PCR—SSP法扩增ABO血型等位基因。结果 一家5人中,兄弟3人均为类孟买型,ABO基因分型分别为A102B、A102B、Al20O1,而其父母血型基因与血清学血型相符合。结论 ABO PCR—SSP基因分型是一种方便、快速、可靠的技术,与血型血清学相比有着显优势。

关 键 词:基因分型技术 类孟买型 家系 ABO基因 基因多态性 PCR-SSP法
文章编号:1009-3257(2003)02-0005-03
修稿时间:2003-01-24

Genotyping of ABO blood group in a family of para-Bombay type in Chinese firstly
YU Qiong,WU Guo-guang,ZHAOTong-mao,et al.. Genotyping of ABO blood group in a family of para-Bombay type in Chinese firstly[J]. Journal of Rare and Uncommon Diseases, 2003, 10(2): 5-7
Authors:YU Qiong  WU Guo-guang  ZHAOTong-mao  et al.
Affiliation:YU Qiong,WU Guo-guang,ZHAOTong-mao,et al. Shenzhen Institute of Transfusion Medicine,Shenzhen 518035,China
Abstract:ObjectiveTo investigate the significance of ABO genotyping technique in clinical transfusion.MethodsToreport the research of ABO genotyping in a family of five people some of which are para-Bombay type in Chinese fristly.Afterserologic tests including absorption-elution tests,saliva neutralizing or inhibitor substances tests and etc were used,we studiedthe polymerase chain reaction with sequence-specific primers(PCR-SSP)based ABO genotyping using 7 designed primers toamplify ABO gene when DNA was extracted from peripheral blood by rapid salt method.ResultsThree children were para-Bombay types.The ABO types were A102B,A102B and A102O1.The genotypes of father and mother accorded with serologicaltypes.ConclusionsABO genotyping technique is a simple rapid and accurate method for determining the difficult case ofABO typing that often happens at the reference laboratory when the serologic tests are used to determine the ABO types ofindividuals.
Keywords:para-Bombay type  PCR-SSP  ABO genotyping  
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号