An ornithine decarboxylase activity assay to screen for potential chemopreventive agents |
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| Authors: | Sharma Sheela Stutzman Jill D. Garris Kyle R. Steele Vernon E. |
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| Affiliation: | (1) Cellular and Molecular Toxicology Program, ManTech Environmental Technology Inc., Research Triangle Park, North Carolina, USA;(2) Chemoprevention Branch, Division of Cancer Prevention and Control, National Cancer Institute, Bethesda, Maryland, USA |
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| Abstract: | A unique assay method to detect ornithine decarboxylase (ODC) activity in normal tracheal epithelial cells induced by a classic tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA) has been developed. Using this new assay, the inhibition of induced ODC activity is measured, thereby providing a screening system to identify potential chemopreventive or therapeutic agents. The assay is designed to be specific as it detects a radioactively labeled reaction product, putrescine from the decarboxylation of ornithine by ODC. As TPA is a classical tumor promoter, inhibition TPA-induced ODC activity can also be used to select antitumor promoter agents. Since the assay requires only 2 days to complete, it can be used to screen a large number of compounds for chemopreventive activity in a relatively short period of time. |
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| Keywords: | Chemopreventive agent Ornithine decarboxylase Tracheal epithelial cells Tumor promoter |
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