| 抗-HCV酶免试剂用于血站血液筛查的效果评价 |
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| 引用本文： | 邱艳,高波,叶建伟,龚小燕,高国静,王全立. 抗-HCV酶免试剂用于血站血液筛查的效果评价[J]. 临床输血与检验, 2003, 5(2): 88-90 |
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| 作者姓名： | 邱艳 高波 叶建伟 龚小燕 高国静 王全立 |
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| 作者单位： | 1. 100088,北京市红十字血液中心
2. 军事医学科学院野战输血研究所 |
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| 基金项目： | 北京市 2 0 0 1年科技新星计划资助 |
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| 摘 要： | 目的 对目前国内血站采用的抗—HCV酶免试剂进行效果评价，为血站合理选用抗—HCV筛查试剂提供参考依据。方法 对不同厂家抗-HCV酶免试剂检测阳性标本分别用抗—HCV确认实验及逆转录—巢式PCR(RT—nPCR)技术进行检测，并对结果进行比较分析。结果 不同厂家抗—HCV酶免试剂对HCV的C区和NS5区抗体检测结果的差异无显性；但对NS3和NS4区抗体检测结果的差异有显性。结论 血站应根据不同抗—HCV试剂对HCV不同区段抗体的检出特性合理选择初复检试剂。
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| 关 键 词： | 抗－HCV酶免试剂 血站 血液筛查 效果 评价 丙型肝炎 |
| 文章编号： | 1671-2587(2003)02-0088-04 |
| 修稿时间： | 2002-10-18 |
Evaluation of anti-HCV EIA kits on the screening of blood |
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| QIU Yan,GAO Bo,YE Jianwei,et al.. Evaluation of anti-HCV EIA kits on the screening of blood[J]. Journal of Clinical Transfusion and Laboratory Medicine, 2003, 5(2): 88-90 |
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| Authors: | QIU Yan GAO Bo YE Jianwei et al. |
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| Affiliation: | QIU Yan,GAO Bo,YE Jianwei,et al.Beijing Red Cross Blood Center,Beijing 100088 |
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| Abstract: | Objective To evaluate the anti HCV enzyme immunoassay (EIA) kits on the screening of blood donors and blood products.Methods The positive samples detected with different EIA kits were analyzed again with anti HCV confirmation tests and RT nPCR.Results There was no significant difference on the detection of antibodies from regions of HCV core and NS5 among different kits;but there was significant difference on detection of antibodies from regions of HCV NS3 and NS4.Conclusion The evaluation and selection of anti HCV EIA kits should be carried out in blood centers according to the different characterization of different anti HCV kits,so as to escape the HCV transmission by blood transfusion. |
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| Keywords: | Hepatitis C Diagnostic kit Enzyme immunoassay Quality control |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
