种植体周龈沟液高迁移率族蛋白B1的检测分析

目的:检测种植体周围炎患者龈沟液(gingival crevicular fluid,GCF)中高迁移率族蛋白B-1(high mobility groupbox l,HMGB-1)的水平,以探讨HMGB-1水平与种植体周围炎间的关系。方法 :经患者知情同意,选取行种植手术1年以上的健康种植体39颗和种植体周围炎40颗,共79颗种植体,收集种植体周围GCF,并运用ELISA法检测其中HMGB-1、IL-1β、IL-8、TNF-α的浓度,同时检测种植体周围的临床指标。结果 :轻度种植体周围炎组的HMGB-1、IL-1β、IL-8及TNF-α浓度高于健康种植体组,重度种植体周围炎的浓度水平又高于轻度种植体周围炎组,HMGB-1与IL-1β、TNF-α的浓度之间亦存在正相关关系(P<0.01)。结论:种植体周围GCF中HMGB-1水平变化与种植体周围炎的病变程度有关,且与经典炎症因子间存在显著相关性,临床检测GCF中HMGB-1水平可作为诊断种植体周围炎的客观指标。

Expression of High Mobility Group Box 1 Levels in Crevicular Fluid of Peri-implantitis Tissues

Objective：To analyze whether the expression of high mobility group box 1 protein （HMGB-1） in gingival crevicular fluid （GCF） are associated with the severity of peri-implantitis. Methods：GCF was sampled from 79 implantation patients including 39 normal peri-implant tissue and 40 peri-implantitis tissues. HMGB-1 ,IL-1β,IL-8,and TNF-α levels were analyzed using ABC-ELISA. Clinical parameters were measured to investigate their relationships. Statistical analyses were performed with SPSS 13.0 software. Results： Significant difference of HMGB-1 expression was observed between healthy implant sites and peri-implantitis sites （P〈0.01）. The expression levels of HMGB-1 showed positive correlation with those of IL-lβ ,IL-8, and TNF-α. Conclusion： HMGB-1 cytokine in GCF may be of value as a parameter in diagnosis and prognosis for peri-implantitis.