感染骨灭活原位移植自体富血小板血浆复合骨髓治疗慢性骨髓炎

目的:评价感染骨灭活原位移植自体富血小板血浆复合骨髓治疗慢性骨髓炎的疗效.方法:40只新西兰大白兔,随机分成两组,制成慢性骨髓炎模型.以感染灶最严重处为中心,线锯截取出死骨及感染骨2.0 cm煮沸灭活15 min,原位移植并闭合伤口.实验组术后局部注射制备好的自体富血小板血浆复合骨髓悬液,隔周1次,连续注射3周.对照组注射生理盐水.分别于术后第4、8、12、16周处死5只动物,行X线观察、组织学观察.结果:实验组成骨修复X线评分、组织学评分均高于对照组,两组之间差异有显著性(P<0.05).结论:感染骨灭活原位移植可作为骨支架,自体富血小板血浆复合骨髓,具有抗感染及促进成骨的双重作用,治疗慢性骨髓炎效果良好.

The study of orthotopic transplantation by inactivated infection bone and autologous platelet-rich plasma combined with bone marrow on chronic osteomyelitis

Objective To evaluate the treatment effect of orthotopic transplantation by inactivated infection bone and autologous platelet-rich plasma combined with bone marrow on chronic osteomyelitis.Methods Forty New Zealand white rabbits were randomly divided into two groups.A model of chronic osteomyelitis was performed.At the center of the most serious foci,the sequestrum and infected bone(2.0 cm) were removed,which boiled for 15 minutes.Then the orthotopic transplantation was performed,the wound was closed.The treatment group was injected with autologous platelet-rich plasma combined with bone marrow suspension in local foci after the operation,once every other week,continuous 3 weeks.The control group was injected with normal saline.Five animals were sacrificed respectively after 4,8,12,16 weeks.The treatment effect was evaluated by radiographs and histological analysis.Results Compare with control group,osteoblast repaired X-ray scores,histological scores of bone repaired in the treatment group were significance increased.The result has statistical significance(P < 0.05).Conclusions Orthotopic transplantation of inactivated infection bone can be used as bone scaffold.Otherwise,autologous platelet-rich combined with bone marrow had the dual roles of anti-infection and promoting bone formation,which have a good effect on chronic osteomyelitis.