PTEN、P27基因甲基化及蛋白表达与子宫内膜癌发生发展的关系

目的:探讨PTEN及P27基因甲基化状态及其蛋白表达与子宫内膜癌发生发展的关系。方法:用甲基化特异性PCR检测36例子宫内膜癌、17例子宫内膜增生症及27例正常子宫内膜组织中的PTEN及P27基因甲基化情况;应用免疫组化Elivision二步法检测PTEN、P27基因在以上3种组织中蛋白表达情况。结果:在子宫内膜癌及子宫内膜增生症中PTEN及P27基因甲基化率明显高于正常子宫内膜(P<0.05),在子宫内膜癌中PTEN及P27蛋白表达率明显低于子宫内膜增生症及正常子宫内膜(P<0.05)。同时,PTEN蛋白表达与P27蛋白表达呈正相关(r=0.491,P=0.005)。结论:PTEN及P27基因甲基化可能参与子宫内膜癌的发生发展;P27基因甲基化可能是子宫内膜癌早期分子事件,可作为子宫内膜癌发生的预测及预防的靶点;在子宫内膜癌中,PTEN蛋白与P27蛋白表达间存在正向调控关系。

The Relationship between Tumor Suppressor Gene Methylation of PTEN,P27 and Carcinogenesis of Endometrial Carcinoma

Objective:To explore the role of DNA methylation and protein expression of PTEN and P27 in the carcinogenesis of endometrial carcinoma.Methods:PTEN and P27 gene methyaltion status were dectected with methylation specific PCR(MSP) and the corresponding protein expressions were measured by immunohistochemistry in 36 cases of endometrial carcinoma,17 cases of endometrial hyperplasia and 27 cases of normal endometrium.Results:The methylation rates of PTEN and P27 in endometrial carcinoma and hyperplasia were significantly higher than those in normal endometrium tissue(P<0.05).The protein expressions of PTEN and P27 in endometrial carcinoma were significantly lower than those in normal endometrium and hyperplasia(P<0.05).There was a positive correlation between PTEN and P27 protein expression(r=0.491,P=0.005).Conclusions:PTEN and P27 methylation may play an important role in the carcinogenesis and development of endometrial carcinoma.P27 gene methylation may be an early molecular event in endometrial carcinoma,which can be used as a predictive or preventive target for endometrial carcinoma.There were a positive correlation between PTEN and P27 protein expression.