| 表面等离子体共振生物传感器在卵巢肿瘤标志物CA125检测中的应用 |
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| 引用本文： | 王耀玲,王瑞娟,王亚琴.表面等离子体共振生物传感器在卵巢肿瘤标志物CA125检测中的应用[J].陕西医学杂志,2012,41(2):212-214. |
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| 作者姓名： | 王耀玲 王瑞娟 王亚琴 |
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| 作者单位： | 1. 西安市第二医院妇产科 西安710003
2. 西安市第二医院妇产科 西安710003 ;陕西省人民医院妇产科 |
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| 基金项目： | 西安市科技计划资助项目 |
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| 摘 要： | 目的:运用表面等离子体共振生物传感器(SPR)无标记检测CA125血清抗原,探索卵巢癌无创诊断方法。方法:采用分子自组装技术,在SPR生物传感片表面固定抗CA125单克隆抗体OC125,利用其检测人体血清中CA125抗原的表达。结果:SPR对血清中CA125的测量结果与放射免疫测量结果的变化趋势基本一致,SPR共振峰移动的大小(δλ)相对地反映了血清中CA125抗原表达程度。结论:卵巢癌患者血清的SPR共振峰波长移动值显著大于对照组血清的SPR共振峰波长移动值。与现有检测技术相比,SPR方法具有无需标记,操作简单等优点,便于基层医疗单位使用。
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| 关 键 词： | 卵巢肿瘤/诊断 CA-125抗原 @表面等离子体共振生物传感器 |
Application of surface plasmon resonance biosensor in detection of ovarian tumor marker CA125 |
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| Wang Yaoling,Wang Ruijuan,Wang Yaqing.Application of surface plasmon resonance biosensor in detection of ovarian tumor marker CA125[J].Shaanxi Medical Journal,2012,41(2):212-214. |
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| Authors: | Wang Yaoling Wang Ruijuan Wang Yaqing |
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| Abstract: | Objective:Explore a label free method for CA125 antigen detection in human serum for clinical diagnosis of ovarian cancer.Methods:Surface plasmon resonance biosensor(SPR)was used and 15 case of human sera were measured using SPR method.The specimens were also measured using RIA method for comparison.Results:SPR results were relatively according with that of RIA method.Conclusion:SPR technology can be used as label free method in ovarian cancer marker CA125 detection. |
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| Keywords: | Ovarian neoplasms/diagnosis CA-125 Antigen @Surface plasmon resonance biosensor |
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