| Leptin促进人脑胶质瘤U87MG细胞的迁移和侵袭 |
| |
| 引用本文： | 韩国胜,刘建民,岳志健,胡小吾,王来兴.Leptin促进人脑胶质瘤U87MG细胞的迁移和侵袭[J].中国肿瘤生物治疗杂志,2012,19(2):154-157. |
| |
| 作者姓名： | 韩国胜 刘建民 岳志健 胡小吾 王来兴 |
| |
| 作者单位： | 第二军医大学附属长海医院神经外科,上海,200433 |
| |
| 基金项目： | 国家自然科学基金资助项目（No. 81101906)；上海市科委基金资助项目（No. 8411953600) |
| |
| 摘 要： | 目的:探讨瘦素(leptin)对人脑胶质瘤U87MG细胞迁移及侵袭能力的影响及其机制。方法:Leptin处理U87MG细胞,采用细胞划痕实验检测U87MG细胞的迁移能力,Transwell实验检测U87MG细胞的侵袭能力,RT-PCR及Western blot-ting法检测U87MG细胞中MMP-2及MMP-9 mRNA和蛋白的表达。结果:Leptin明显促进U87MG细胞迁移能力(152.42±3.29)vs(83.24±2.61)μm,P<0.05]和侵袭能力(31.78±5.04)vs(17.03±2.41)个细胞,P<0.05],leptin能显著上调U87MG细胞中MMP-2、MMP-9 mRNA(0.76±0.04)vs(0.35±0.02),(0.84±0.02)vs(0.41±0.06);均P<0.05]及蛋白(0.79±0.03)vs(0.23±0.01),(0.81±0.05)vs(0.39±0.03);均P<0.05]的表达。MMP抑制剂GM6001(10μmol/ml)可以逆转leptin对U87MG细胞迁移(82.05±2.98)vs(81.76±3.25)μm,P>0.05]和侵袭能力(19.23±2.46)vs(18.02±1.98)个细胞,P>0.05]的影响。结论:Leptin可以促进人脑胶质瘤U87MG细胞的侵袭及迁移,其机制可能与上调MMP-2、MMP-9的表达有关。
|
| 关 键 词： | 瘦素 人脑胶质瘤 U87MG细胞 MMP-2 MMP-9 迁移 侵袭 |
| 收稿时间： | 2011/12/10 0:00:00 |
| 修稿时间： | 2012/1/25 0:00:00 |
Leptin promotes migration and invasion of human glioma U87MG cells |
| |
| HAN Guo-sheng,LIU Jian-min,YUE Zhi-jian,HU Xiao-wu and WANG Lai-xing.Leptin promotes migration and invasion of human glioma U87MG cells[J].Chinese Journal of Cancer Biotherapy,2012,19(2):154-157. |
| |
| Authors: | HAN Guo-sheng LIU Jian-min YUE Zhi-jian HU Xiao-wu and WANG Lai-xing |
| |
| Institution: | Department of Neurosurgery, Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433, China;Department of Neurosurgery, Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433, China;Department of Neurosurgery, Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433, China;Department of Neurosurgery, Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433, China;Department of Neurosurgery, Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433, China |
| |
| Abstract: | Objective: To investigate the effect of leptin on the migration and invasion of human glioma U87MG cells,and explore its molecule mechanism.Methods: U87MG cells were treated with leptin,the cell migration was analyzed by cell scratch assay,cell invasion was detected by Transwell,and mRNA and protein expressions of MMP-2 and MMP-9 in U87MG cells were observed by RT-PCR and Western blotting assay.Results: Leptin significantly promoted migration(152.42±3.29] vs 83.24±2.61] μm,P<0.05),and invasion(31.78±5.04] vs 17.03±2.41]/field,P<0.05) of U87MG cells;the mRNA and protein expressions of MMP-2 and MMP-9 were significantly upregulated by leptin in U87MG cells(mRNA: 0.76±0.04] vs 0.35±0.02],0.84±0.02] vs 0.41±0.06],P<0.05;protein: 0.79±0.03] vs 0.23±0.01],0.81±0.05] vs 0.39±0.03],P<0.05);MMP inhibitor GM6001 reversed U87MG cell migration(82.05±2.98] vs 81.76±3.25] μm,P>0.05] and invasion(19.23±2.46] vs 18.02±1.98]/field,P<0.05) induced by leptin.Conclusion: Leptin can promote the invasion and migration of human glioma U87MG cells,which might related with the upregulation of MMP-2 and MMP-9. |
| |
| Keywords: | leptin human glioma U87MG cell MMP-2 MMP-9 migration invasion |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国肿瘤生物治疗杂志》浏览原始摘要信息 |
| 点击此处可从《中国肿瘤生物治疗杂志》下载免费的PDF全文 |
|
