血红素加氧酶-1启动子基因多态性与高血压的关系

目的:探讨血红素加氧酶-1(HO-1)基因启动子区域的(GT)n双核苷酸重复序列多态性与天津地区原发性高血压(EH)的关系及与血浆炎症因子、胆红素水平的关联性.方法:选择102例EH患者(EH组)及134例正常对照组作为研究对象,采用聚合酶链反应(PCR)方法扩增染色体DNA中含HO-1基因相应的DNA片段,聚丙烯酰胺凝胶电泳(PAGE)技术分析基因型.自动生化分析仪分析血浆胆红素水平.酶联免疫吸附(ELISA)法分析血清白细胞介素(IL)-10及肿瘤坏死因子(TNF)-α水平.结果:EH组血浆胆红素、TNF-α、IL-10水平显著高于对照组,差异有统计学意义(P＜0.05).HO-1重复≥32次长等位基因在EH组的分布明显高于对照组,差异有统计学意义(P＜0.01).HO-1长等位基因携带者血浆胆红素水平低于短等位基因携带者,差异有统计学意义(P＜0.05).结论:HO-1基因启动子区域的(GT)n双核苷酸重复多态性可能与EH人群的易感性有关.HO-1长等位基因携带者患高血压的危险性增大.

The Relationship between Heme Oxygenase-1 Promoter Polymorphism and Hypertension

Objective:To investigate the relationship between the length polymorphism of GT repeat in heme oxygenase-1(HO-1) promoter area and essential hypertension(EH),and the correlation of this polymorphism with plasma levels of inflammatory cytokines and bilirubin.Methods:The HO-1 gene GT dinucleotide repeat polymorphism in promoter area was evaluated in 102 EH patients and 134 healthy controls.The genotypes were analyzed by PCR and polyacrylamide gel electrophoresis.The bilirubin level was measured by automatic chemistry analyzer.The serum levels of TNF-α and IL-10 were detected by ELISA method.Results:The plasma levels of bilirubin,TNF-α and IL-10 were significantly higher in EH group than those of control group(P < 0.05).The long allele of HO-1 with ≥32(GT) repeats(L allele) was found more frequently in EH group than that of control group(P < 0.01).The plasma bilirubin levels in patients carrying with long allele HO-1 were lower than the cases carrying with short allele(P < 0.05).Conclusion:The long allele of HO-1 genotype may be associated with the susceptibility of essential hypertension.Patients with HO-1 long allele have an increased risk of high blood pressure.