血卟啉单甲醚对人宫颈癌Hela细胞体外影响的研究

目的:初步探讨血卟啉单甲醚(hematoporphyrin monomethyl ether,HMME)介导的光动力疗法(HMME-PDT)对体外培养的人宫颈癌Hela细胞的杀伤效应及影响宫颈癌细胞光动力疗法(photodynamic therapy,PDT)杀伤效应的主要因素。方法:通过采用不同光敏剂浓度、光照能量密度及细胞孵育时间介导的光动力作用于人宫颈癌Hela细胞,MTT法检测24h后的细胞生长抑制率。结果:光敏剂浓度<2.5μg/mL的不同组细胞生长抑制率有明显差异,光照能量密度≥1.5J/cm2及孵育时间≥4h的细胞之间生长抑制率差异无统计学意义。HMME 2.5μg/mL、光照能量密度1.5J/cm2和孵育时间≥4h可能是PDT对Hela细胞杀伤作用的最佳参数。结论:特定光源下,光敏剂的浓度、光照剂量及孵育时间是影响HMME-PDT效应的主要因素。

Effects of hematoporphyrin monomethyl ether on human cervical carcinoma Hela cell lines

Objective To investigate the lethal effects of hematoporphyrin monomethyl ether(HMME) mediated photodynamic therapy(PDT) on human cervical carcinoma Hela cell lines and the factors affecting these effects.Methods Hela cells were treated with different concentrations of HMME,different energy density of laser,and different incubation time,then the survival rate of Hela cells was measured by MTT assay 24 h later.Results There were significant differences of cell growth inhibition among the groups with concentrations of HMME less than 2.5 μg /mL,but there were no significant differences among the groups with laser energy density more than 1.5 J /cm2 and incubation time more than 4 h.The parameters of 2.5 μg /mL of HMME,1.5 J /cm2 of laser energy density,and more than 4 h of incubation time were the best for the lethal effects of HMME-PDT on Hela cells.Conclusions The main impact factors of HMME-PDT include concentrations of HMME,laser energy density,and incubation time.