| IL-18和HIV-1 gag-gp120嵌合基因DNA疫苗联合免疫小鼠的免疫应答 |
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| 引用本文： | 江文正,金宁一,李子健,张立树,王宏,金洪涛.IL-18和HIV-1 gag-gp120嵌合基因DNA疫苗联合免疫小鼠的免疫应答[J].细胞与分子免疫学杂志,2004,20(4):433-435. |
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| 作者姓名： | 江文正 金宁一 李子健 张立树 王宏 金洪涛 |
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| 作者单位： | 1. 解放军军需大学全军基因工程重点实验室,吉林,长春,130062
2. 吉林大学第一医院,吉林,长春,130023 |
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| 基金项目： | 国家杰出青年科学基金资助 (No .3982 51 1 9)，国家高技术研究发展计划 (863)资助项目 (No.2 0 0 1AA2 1 50 31 ) |
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| 摘 要： | 目的 :探讨IL 18和HIV 1gag gp12 0嵌合基因的DNA疫苗联合免疫小鼠的免疫应答。方法 :构建含IL 18的真核表达质粒pVAXIL18,将他与表达HIV 1gag gp12 0嵌合基因的核酸疫苗质粒pVAXGE共同肌注免疫BALB/c小鼠 ,检测免疫小鼠脾特异性CTL杀伤活性和血清抗体滴度。结果 :联合免疫组小鼠脾特异性CTL杀伤活性和血清抗体水平均显著高于单独免疫组 (P <0 .0 5 ) ,空白质粒对照组 (P <0 .0 1)和PBS对照组 (P <0 .0 1)。结论 :IL 18和HIV 1gag gp12 0嵌合基因的DNA疫苗联合免疫可诱导小鼠产生特异性细胞和体液免疫 ,且IL 18发挥了免疫佐剂的作用。
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| 关 键 词： | HIV-1 gag-gp120 IL-18 DNA疫苗 |
| 文章编号： | 1007-8738(2004)04-0433-00 |
| 修稿时间： | 2003年6月26日 |
The immune response in mice inoculated with HIV-1 gag-gp120 chimeric gene DNA vaccine and IL-18 plasmid |
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| JIANG Wen-zheng,JIN Ning-yi ,LI Zi-jian,ZHANG Li-shu,WANG Hong,JIN Hong-tao Key Laboratory of Genetic Engineering,Quartermaster University of PLA,Changchun ,First Hospital,Jilin University,Changchun ,China.The immune response in mice inoculated with HIV-1 gag-gp120 chimeric gene DNA vaccine and IL-18 plasmid[J].Journal of Cellular and Molecular Immunology,2004,20(4):433-435. |
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| Authors: | JIANG Wen-zheng JIN Ning-yi LI Zi-jian ZHANG Li-shu WANG Hong JIN Hong-tao Key Laboratory of Genetic Engineering Quartermaster University of PLA Changchun First Hospital Jilin University Changchun China |
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| Institution: | Key Laboratory of Genetic Engineering, Quartermaster University of PLA, Changchun 130062, China. wzjiang@hotmail.com |
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| Abstract: | AIM: To explore the immune response in mice inoculated with HIV-1 chimeric gene gag-gp120 DNA vaccine and IL-18 plasmid. METHODS: BALB/c mice were injected i.m with eukaryotic expression plasmid pVAXIL18 containing IL-18 gene and DNA vaccine plasmid pVAXGE containing HIV-1 gag-gp120 chimeric gene. The specific killing activities of spleen CTL and the titers of serum antibodies of the immunized mice were detected. RESULTS: The specific killing activities of spleen CTL and the titers of serum antibodies in the co-inoculation group were significantly higher than those in the single inoculation group(P<0.05), the vector control group (P<0.01) and PBS control group (P<0.01). CONCLUSION: The specific cellular and humoral immunity in mice can be induced by co-inoculating HIV-1 gag-gp120 chimeric gene DNA vaccine and IL-18 plasmid indicating that IL-18 augments immune response as immunoadjuvant. |
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| Keywords: | HIV-1 gag-gp120 IL-18 DNA vaccine |
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