应用酶联免疫吸附试验检测肠出血性大肠杆菌O157:H7

目的制备和纯化O157：H7抗原，免疫家兔和豚鼠，获得高效价的抗O157：H7免疫血清并进行纯化及酶标记。建立对O157：H7感染患者快速诊断方法，做到早期发现及时治疗，有效控制疫情的蔓延。方法ELISA双抗体夹心法检测O157：H7抗原。步骤：包被特异性抗体，加处理后的粪便等标本，然后加入抗O157：H7酶结合物，最后加入底物显色。结果本课题所研制的抗O157：H7酶结合物只对O157：H7呈阳性反应，而与其他相关细菌无交叉反应。结论应用酶联免疫吸附试验(即双抗体夹心法)对肠出血性大肠杆菌O157：H7抗原的检测较常规法实验程序简捷、快速、敏感。临床和现场验证结果表明，其方法具有灵敏度高，特异性强操作简单等特点，为肠出血性大肠杆菌O157：H7的鉴定及快速诊断提供了一种新的检测手段。

Detection of the intestinal hemorrhage colon bacillus O157:H7 by ELISA

Objective To establish an immunity method for rapid diagnosis of O157:H7 infection, in order to take effective control for epidemic spread. Methods The high titer immune serum was obtained by immunizing the guinea pig and rabbit immunized with O157:H7 antigens, and then treated by purification and enzyme labelling. The O157:H7 antigen was wrapped up, and added with treated excrement specimen as well as the combination of O157:H7 antibody and ferment. The method of double-antibody ELISA was used to analyze the treated O157:H7 antigen. Results The combination united O157:H7 antibody and ferment only appeared positive reaction to O157:H7, but no consensual reaction with other germs. Conclusion The double-antibody ELISA is a forthright, quick, and sensitive method for detecting the intestinal hemorrhage colon bacillus O157:H7, which offers a new analysis method for appraisal and diagnose the intestinal hemorrhage colon bacillus O157:H7