Genetic diversity of G9 rotavirus strains circulating among diarrheic children in North India: A comparison with 116E rotavirus vaccine strain |
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| Authors: | Shipra Gupta Vasundhara Razdan Tiku Mariyam Gauhar Kahkashan Khatoon Pratima Ray |
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| Affiliation: | 1. Department of Biotechnology, School of Chemical & Life Sciences, Jamia Hamdard, New Delhi 110062, India;2. Department of Pediatrics, All India Institute of Medical Sciences, New Delhi 110023, India |
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| Abstract: | The parental rotavirus strain 116E (G9P[11]) used to generate Rotavac® vaccine was isolated in 1986 in New Delhi. Thenceforward, there is no comprehensive report on diversity of G9 rotavirus strains from 116E; therefore, the present study evaluates the VP7 gene sequence diversity of G9 strains (retrieved from GenBank) from different geographical regions (1987–2016). Additionally, 22 recently collected G9 strains from Himachal Pradesh and Delhi (2013–2016) were included in the phylogenetic analysis. Interestingly, unlike 116E which belong to lineage-II all other G9 rotavirus including these 22 samples clustered together in a separate lineage (III). Further, six amino acid substitutions including one novel, K143M (epitope 7-2) different from 116E were detected mostly in the neutralization epitopes of VP7 protein (neutralization escape mutants). Overall, the accumulation of identified substitutions in VP7 epitopes and evolution of G9 strains in India may have impact on Rotavac® efficacy. |
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| Keywords: | Rotavirus VP7 gene G9 Rotavac® 116E Diarrhea Children Antigenic epitopes Vaccine Phylogenetic analysis RVs" },{" #name" :" keyword" ," $" :{" id" :" k0060" }," $$" :[{" #name" :" text" ," _" :" rotaviruses AGE" },{" #name" :" keyword" ," $" :{" id" :" k0070" }," $$" :[{" #name" :" text" ," _" :" acute gastroenteritis HP" },{" #name" :" keyword" ," $" :{" id" :" k0080" }," $$" :[{" #name" :" text" ," _" :" Himachal Pradesh |
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