Use of MALDI-TOF MS (Bruker Daltonics) for identification of Mycobacterium species isolated directly from liquid medium |
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| Authors: | Marta Fernández-Esgueva Rebeca Fernández-Simon María Luisa Monforte-Cirac Ana Isabel López-Calleja Blanca Fortuño Jesús Viñuelas-Bayon |
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| Institution: | 1. Servicio de Microbiología, Hospital Universitario Miguel Servet. IIS Aragón, Zaragoza, Spain;2. Grupo de estudio de las Infecciones por Micobacterias (GEIM), Sociedad Española de Microbiología y Enfermedades Infecciosas, Spain |
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| Abstract: | ObjectivesThe aim of this study was to describe the evaluation of the use of MALDI-TOF MS for the identification of non-tuberculous mycobacteria (NTM) and Mycobacterium tuberculosis directly from liquid MGIT cultures from January 2017 to December 2017.Material/methodsA total of 155 isolates (mainly respiratory) were analyzed by MALDI-TOF MS (Bruker Daltonics) directly from MGIT liquid medium with a previous extraction procedure.ResultsMALDI-TOF MS generated acceptable scores for 152 isolates (98.06%). Fifty isolates were identified as M. tuberculosis complex and the remaining 105 as NTM (M. abscessus subsp. abscessus, M. avium, M. celatum, M chelonae, M. chimaera, M. fortuitum, M. gordonae, M. intracellulare, M. kansasii, M. lentiflavum, M. mageritense, M. mucogenicum and M. xenopi).ConclusionsThese results indicate that MALDI-TOF MS can be useful to identify mycobacteria directly from MGIT cultures and is an accurate, rapid and cost-effective system to be used as a routine method. |
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| Keywords: | Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) Mycobacteria MGIT liquid medium Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) Micobacteria Medio líquido MGIT |
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