抑肽酶对脑出血后血肿周围组织细胞凋亡的干预研究

目的:探讨脑出血后血肿周围组织细胞凋亡的变化规律,及抑肽酶的作用。方法:尾状核注射胶原酶诱导大鼠脑出血模型,用TUNEL染色法观察血肿周围细胞凋亡的发生,用免疫组化法观察半胱氨酰天冬氨酸蛋白酶一3 (caspase-3)的蛋白表达,以及抑肽酶的作用。结果:脑出血后可见血肿周围带有较多的caspase-3免疫阳性细胞和凋亡细胞,前者的表达高峰在造模后48h,后者的表达高峰在造模后72h,两者的表达规律类似;抑肽酶干预组的阳性细胞效显著减少,染色变浅。结论:抑肽酶能够明显减轻脑出血后的细胞凋亡,可能与抑制caspase-3的表达有关。

The Intervension Study of Apretinin on Apoptosis in Perihematoma Tissue after lntracerebral Hemorrhage

Objective: To investigate the dynamic changes of apoptosts around hematoma in acute intracerebral hemor-rhage(ICH). Method: Intracerebral hemorrhagic model was induced utilizing the local injection of collagenase into the basal ganglia with sterile technique, apoptosis levels were detected by TUNEL technique, the changes of caspase-3 were detected by immunohistochemistry, meanwhile the intervension effect of aprotinin was observed. Result: Many caspase-3 and TUNEL positive ceils appeared in perihematoma tissue, the number of positive caspase-3-immunostaining ceils peaked at 48h while apoptosis ceil at 72h after hemorrhage, their expressions were similar! the positive ceils decreased and became weaker in aprotinin-treated group. Conclusion: Aprotinin could greatly reduced the amount of apoptosis ceils after ICH, which probably related inhibiting caspase-3 expression.