乳腺癌中COX-2表达及其抑制剂塞来昔布对乳腺癌细胞系增生和凋亡的影响

目的为进一步观察COX-2对乳腺癌的作用，我们检测了乳腺癌组织、乳腺癌细胞株MCF-7和B37中COX-2的表达，并观察选择性COX-2抑制剂塞来昔布对乳腺癌细胞株生长和凋亡的影响。方法应用免疫组织化学染色的方法检测132例乳腺癌组织标本中COX-2蛋白的表达；应用免疫细胞化学染色、原位杂交、逆转录多聚酶联反应（RT-PCR）的方法，分别检测COX-2蛋白和mRNA在乳腺癌细胞株MCF-7、B37中的表达；采用MTT法、AO/EB双荧光染色法和流式细胞术，研究塞来昔布对乳腺癌细胞增殖和凋亡的影响。结果52.3%(69/132)的乳腺癌组织标本表达COX-2蛋白；在乳腺癌细胞株MCF-7和B37中均有COX-2的表达，25μmol/L塞来昔布作用72h后，COX-2表达明显减少；25、50、100μmol/L的塞来昔布与MCF-7细胞作用72h，生长抑制率分别为36.3%、57.7%、74.5%。100μmol/L塞来昔布作用72h后，MCF-7细胞凋亡率为38.5％。结论乳腺癌组织和乳腺癌细胞株MCF-7、B37中存在COX-2的表达；塞来昔布可抑制乳腺癌细胞增殖，并促进细胞凋亡，塞来昔布可能作为新的药物治疗乳腺癌。

Expression of Cyclooxygenase-2(COX-2)in Breast Cancer and Effects of COX-2 Inhibitor Celecoxib on Proliferation and Apoptosis of Breast Cancer Cell Lines

Objective To approach whether Celeoxib could be used to cure breast cancer,the protein and mRNA level of COX-2 in breast cancer tissues and breast cancer cell lines MCF-7 and B37 was detected,and MCF-7 cells were cultured with Celecoxib to observe the effect of Celecoxib on proliferation and apoptosis of breast cancer cell lines. Methods Immunohistochemical staining,in situ hybridization and RT-PCR were used to detect the expression of COX-2 protein and mRNA in breast cancer tissues and breast cancer cell lines MCF-7 and B37; MTT assay and AO/EB double staining were used to show effect of Celecoxib on proliferation and apoptosis of breast cancer cell lines. Results COX-2 protein mRNA could be detected in both the breast cancer cell lines MCF-7 and B37,and 52.3% of breast cancer tissues could express COX-2 protein; Celecoxib could inhibited MCF-7 cell growth in a concentration and time dependent manner,and could lead to the 38.5% cell apoptosis of MCF-7 cell. Conclusion Breast cancer cell lines MCF-7,B37 and breast cancer tissues expressed COX-2; Celecoxib could inhibit breast cancer cell proliferation and induce apoptosis,with indicating that Celecoxib can be used as a new chemical drug treatment for breast cancer.