抗DR5单克隆抗体-mDRA-6对白血病细胞的凋亡诱导作用

目的:观察抗死亡受体5(DR5)单克隆抗体(mAb)-mDRA-6对白血病细胞的凋亡作用。方法:DR5蛋白免疫BALB/c小鼠,融合筛选抗DR5杂交瘤细胞,制备抗人DR5mAb-mDRA-6;荧光显微镜下观察mDRA-6作用下白血病细胞Jurkat、HL-60、K562的形态变化;MTT法测定不同浓度的mDRA-6对Jurkat、HL-60、K562细胞存活率的影响;通过FITC-Annexin V及PI标记细胞,以流式细胞术检测mDRA-6对Jurkat、HL-60、K562细胞凋亡率的影响。结果:mDRA-6导致Jurkat、HL-60细胞染色质浓缩、断裂,细胞出芽,凋亡小体形成;mDRA-6对Jurkat、HL-60细胞具有明显的杀伤作用,但对K562的杀伤作用较弱,25mg/L的mDRA-6作用12h,Jurkat、HL-60、K562细胞死亡率分别为88.76%,59.76%,5.18%。Annexin V及PI双染显示1mg/L的mDRA-6作用10h,Jurkat细胞凋亡率为86.06%,10mg/L的mDRA-6作用10h,HL-60细胞凋亡率为48.11%,但10mg/L的mDRA-6作用K562细胞10h,细胞凋亡不明显。结论:抗DR5mAb mDRA-6能够诱导白血病细胞凋亡,不同的白血病细胞株对mDRA-6的敏感性不同。

Leukemic cell apoptosis induced by anti-human DR5 monoclonal antibody mDRA-6

AIM:To investigate the apoptotic effect of anti-human DR5(death receptor 5 of TRAIL)monoclonal antibody mDRA-6 on leukemic cells.METHODS:The morphological changes of leukemic cells were observed by fluorencence microscope.The cytotoxic and apoptotic effects of mDRA-6 on Jurkat,HL-60 and K562 cells were detected by MTT analysis and flow cytometry with Annexin V-FITC/PI staining.RESULTS:Chromatin condensation,budding and apoptotic bodies were observed in Jurkat and HL-60 cells treated by mDRA-6.Death and apoptosis of leukemic cells treated by mDRA-6 were increased,but the effect of mDRA-6 on K562 cells was not obvious.CONCLUSION:Apoptosis of leukemic cells can be induced by anti-human DR5 monoclonal antibody mDRA-6.Different leukemic cell lines are of different sensitivity to mDRA-6.