| 变性高效液相色谱法筛选XRCC4基因突变 |
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| 引用本文： | 贾静,潘建基,苏颖,陈增,邹长棪,陈传本,潘才柱. 变性高效液相色谱法筛选XRCC4基因突变[J]. 福建医科大学学报, 2009, 43(3): 241-243 |
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| 作者姓名： | 贾静 潘建基 苏颖 陈增 邹长棪 陈传本 潘才柱 |
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| 作者单位： | 福建医科大学教学医院、福建省肿瘤医院,放射生物研究室,福州350014 |
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| 摘 要： | 目的应用变性高效液相色谱技术（DHPLC）-异源双链分析对鼻咽癌患者XRCC4基因突变进行筛查与鉴定，研究鼻咽癌患者XRCC4的基因突变情况，探讨DHPLC在筛查相关基因突变、预测放疗敏感性方面的应用。方法采用聚合酶链反应（PCR）和DHPLC筛查88例鼻咽癌患者XRCC4基因的突变。结果对DHPLC图形异常的PCR扩增片断进行DNA测序鉴定突变位点及性质，检测出28例XRCC4基因8号外显子第377位C变成T，导致126号密码子的ser→phe，导致氨基酸残基的替代变化（丝氨酸→苯丙氨酸）。结论用PCR—DHPLC筛查结合测序分析检测XRCC4突变是一种高效、经济、简便、可靠的方法。
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| 关 键 词： | 鼻咽肿瘤 辐射耐受性 突变 肿瘤细胞 培养的 DNA修复 色谱法 高压液相 基因型 |
Screening Gene Mutations of XRCC4 by Denaturing High-performance Liquid Chromatography |
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| JIA Jing,PAN Jianji,SU Ying,CHEN Zeng,ZHOU Changyan,CHEN Chuanben,PAN Caizhu. Screening Gene Mutations of XRCC4 by Denaturing High-performance Liquid Chromatography[J]. Journal of Fujian Medical University, 2009, 43(3): 241-243 |
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| Authors: | JIA Jing PAN Jianji SU Ying CHEN Zeng ZHOU Changyan CHEN Chuanben PAN Caizhu |
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| Affiliation: | JIA Jing,PAN Jianji,SU Ying,CHEN Zeng,ZHOU Changyan,CHEN Chuanben,PAN Caizhu Radiobiology Laboratory,Teaching Hospital of Fujian Medical University,Fujian Provincial Cancer Hospital,Fuzhou 350014,China |
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| Abstract: | Objective To detect mutations in XRCC4 gene in Nasopharyngeal carcinoma(NPC)patients by denaturing high-performance liquid chromatography(DHPLC)and to investigate the potential application of DHPLC in screening gene mutation of XRCC4 for prediction of radio therapy sensitivity.Methods polymerase chain reaction and denaturing high-performance liquid chromatography(DHPLC)were used to screen XRCC4 mutations in 88 patients of NPC.Results The PCR-amplified XRCC4 DNA fragments with a different DHPLC elution profi... |
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| Keywords: | nasopharyngeal neoplasms radiation tolerance mutation tumor cells cultured DNA repair chromatography high pressure liquid genotype |
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