HSP60和CD44 V6在胃腺癌中的表达及其意义

目的研究热休克蛋白60(HSP60)、CD44V6在人胃癌组织中的表达.方法对60例非癌胃粘膜病变及50例胃腺癌组织手术及内镜活检石蜡标本,应用抗人HSP60,CD44V6单克隆抗体,以免疫组织化学检测方法检测HSP60,CD44V6的表达.结果 HSP60在慢性浅表性胃炎、肠上皮化生、不典型增生、胃腺癌的阳性率为30%,65%,70%,76%,过表达率为10%,25%,35%,58%.CD44V6在慢性浅表性胃炎、肠上皮化生、不典型增生、胃腺癌的阳性率为5%,25%,30%,78%,HSP60,CD44V6在肠上皮化生、不典型增生及胃腺癌中的阳性率和过表达率均明显高于慢性浅表性胃炎(P＜0.05);HSP60在胃腺癌组织的过表达率高于肠上皮化生和不典型增生(P＜0.05).HSP60在高、中、低分化胃腺癌中的阳性表达率为64.7%,77.7%,75.0%.HSP60在不同分化程度胃腺癌中表达无明显差异(P＞0.05),而CD44V6在高、中、低分化胃腺癌中的阳性表达率为64.7%,66.2%,91.7%,CD44V6在低分化胃腺癌的表达明显高于高、中度分化胃腺癌(P＜0.05).结论 HSP60,CD44V6在人胃癌及癌前情况或病变中过度表达,可能与胃腺癌的发生、发展有关,还可能有助于预测胃腺癌的分化程度.

Expression of heat shock protein 60,CD44 splice variant V6 in human gastric cancer

AIM To determine the expression of HSP60 and CD_(44)V_6 in human gastric cancer tissues. METHODS Immunohistochemical staining was used in clinical specimens of 50 cases of human gastric cancer and 60 cases of noncancer chronic gastric mucosal lesion with monoclonal antibody against human HSP60,CD_(44)V_6. RESULTS The positive rate of HSP60 in chronic superficial gastritis,intestinal metaplasia,dysplasia and gastric cancer was 30%,65%,70% and 76%,respectively.The positive rate of CD_(44)V_6 in chronic superficial gastritis,intestinal metaplasia,dysplasia and gastric cancer was 5%,25%,30% and 78%.The positive staining rates of HSP60 and CD_(44)V_6 in intestinal metaplasia,dysplasia and gastric cancer were higher than that in chronic superficial gastritis(P<0.05).The overexpression rate of HSP60 in chronic superficial gastritis, intestinal metaplasia,dysplasia and gastric cancer was 10%, 25%,35% and 58%.HSP60 was obviously over-expressed in gastric cancer as compared to that in intestinal and dysplasia (P<0.05).The positive rate of HSP60 in high,moderate and low differentiation of gastric cancer was 64.7%,77.7% and 75.0%.The expressions of HSP60 in variant differentiation of gastric cancers were not different (P>0.05),but the positive rate of CD_(44)V_6 in high,moderate and low differentiation of gastric cancer was 64.7%,66.2% and 91.7%,the expression of CD_(44)V_6 in low differentiation of gastric cancer was more obvious than that in moderate and high differentiation of gastric cancer. CONCLUSION HSP60 and CD_(44)V_6 are expressed in human gastric cancer,which might contribute to the occurrence and development of the cancer.Determination of CD_(44)V_6 by immunohistochemistry might be useful in assessing tumor differentiation.