丹参酮在离体肺保护中应用的实验研究

目的本研究是探讨丹参酮对离体肺保护的作用。方法以离体兔肺模型为肺保护实验对象，以肺动脉灌洗再次肺动脉灌注为实验方法，以新西兰兔为实验动物，随机分为两组。一组用LPD液行肺动脉灌洗和保存．另一组用丹参酮加LPD液组成实验组，用同样的方法灌洗和保存。保存18h后，观察病理形态、含水量以及测量NO（一氧化氮）、SOD（超氧化物歧化酶）及MDA（丙二醛）的含量。结果肉眼见组织新鲜红润，部分组织充血、水肿及淤血；光镜下两组的肺泡、支气管及毛细血管结构完整，高倍视野下对照组较实验组肺泡上皮及毛细血管上皮细胞肿胀、肥大，少部分组织可见肺泡内有红细胞渗出、间隔增宽等。实验组的含水量低于对照组（P〈O．05）。实验组肺组织中的NO及SOD含量高于对照组（P〈O．05）．MDA含量低于对照组（P〈O．05）。结论丹参酮能清除氧自由基，有较强的抗氧化性，能较明显的减轻肺的再灌注损伤。在LPD液中加入丹参酮，能够对离体肺起到更好的保护效果。离体兔肺再灌注模型是一种较理想的实验动物模型。

Study of Proservative Effects of Tanshinone on Isolated-lung

Objective To investigate the preservative effect of tanshinone on isolated lung.Methods Twenty-four New-Zealand rabbits were randomly divided into two groups.In experiment group,the lungs were perfused and preserved with LPD solution,while in control group,the lungs were with LPD+Tanshinone solution.The isolated lungs were preserved for 18 hours and then reperfused with rewarming whole blood.After reperfusing for 30 minutes the lung samples were examined by pathology,wet/dry weight,and NO,SOD and MDA were detected.Results In both two groups the isolated lungs were fresh,but had partial congestion,edema and ecchymoma under naked eye.Under light microscopy the structures of the pulmonary vesiculars,branchia and capillaries were completed,but the epithelial cells of the vesicles and capillaries in the LPD group were more swelling and hypertrophy than that in LPD+Tanshinone group.The wet/dry weight ratio was significantly lower in LPD+Tanshinone group than that in LPD group.The levels of NO and SOD were higher in the LPD+Tanshinone group than that in the LPD group(P<0.05),whole the level of MDA was lower in the LPD+Tanshinonegroup than that in LPD group(P<0.05).conclusions Tanshinone could help in clearing of oxygen free radical and is of stronger antioxidation and result in alleriating of lung reperfusing injury.The morphologic changes between two groups are not significantly different.Tanshinone has positive effect on preservative quality of the isolated lung.The reperfusing model of extracorporeal rabbit lung is a more ideal model.