胃癌组织及转移淋巴组织中胃泌素基因的表达

目的:分析胃癌患者癌组织及转移淋巴结组织中胃泌素基因的表达,进一步阐明胃泌素与胃癌发生、发展的相关机制,为胃癌的防治提供实验依据.方法:收集胃癌手术患者的癌组织、癌旁组织、淋巴结组织,提取及纯化总RNA,采用Taqman实时荧光定量PCR技术测定胃泌素mRNA的表达.结果:胃泌素mRNA表达在胃癌组织较癌旁组织降低,为...

Expression of Gastrin Gene in Human Gastric Cancer Tissues and Metastatic Lymphatic Tissues

Objective:To explore the relationship of expression levels of gastrin gene in matched human gastric carcinoma,lymph tissue and adjacent non-neoplastic mucosa of gastric cancer patients with development and progression of the disease.Methods:Total RNA was extracted and purified from gastric carcinoma tissues,lymph tissue and adjacent non-neoplastic mucosa of patients,and gastrin mRNA levels(mRNAL) were determined with Taqman real-time quantitative PCR.Results:The mRNAL in gastric carcinoma tissues was lower than that in adjacent non-neoplastic mucosa(with ratio of 0.063),and was higher in metastatic lymphatic tissue than that in adjacent non-neoplastic mucosa(with ratio of 35.482).The mRNAL was higher in carcinoma and lymphatic tissues in sinus ventriculi than those in corpus ventriculi with ratioes of 3.074 and 9.190 respectively.The mRNAL was higher in cancer and metastatic lymphatic tissues of metastasis group than those of non-metastatic group with ratioes of 9.912 and 59.912 respectively.The mRNAL was higher in cancer and metastatic lymphatic tissues of group >T2 than those of group ≤T2 with ratioes of 1.540 and 13.833 respectively,and the differences were statistically significant(P<0.01).Conclusions:Gastrin has important significance in the development of gastric cancer.Its expression is highly correlated with situs,metastases and clinical manifestation of gastric cancer.