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| 救必应中长梗冬青苷提取工艺正交试验优化 | |
| 引用本文： | 王海森,李超生,王晶,李喆,于蔷蔷,华山,谷雨. 救必应中长梗冬青苷提取工艺正交试验优化[J]. 中国药师, 2012, 15(6): 805-807 |
| 作者姓名： | 王海森 李超生 王晶 李喆 于蔷蔷 华山 谷雨 |
| 作者单位： | 1. 吉林农业大学,长春,130118 2. 吉林省中医药科学院 3. 延边大学 |
| 基金项目： | 吉林省科技发展计划资助项目（编号：20090927） |
| 摘 要： | 目的:研究长梗冬青苷最佳提取工艺条件.方法:采用正交试验设计研究不同浓度的乙醇、乙醇体积、提取时间和提取次数对长梗冬青苷提取率的影响;采用高效液相色谱法测定提取物中长梗冬青苷含量.结果:救必应中长梗冬青苷最佳提取条件为加入6倍量70%乙醇,回流提取3次,每次1 h.结论:本法提取效率高,适合大量提取,可用于工业化生产. |
| 关 键 词： | 正交试验 救必应 长梗冬青苷 提取工艺 |
| 收稿时间： | 2011-11-19 |
| 修稿时间： | 2012-01-14 |
Extraction Optimization of Pedunculoside in Ilex rotunda Thunb.by Orthogonal Test |
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| Wang Haisen , Li Chaosheng , Wang Jing , Li Zhe , Yu Qiangqiang , Hua Shan , Gu Yu. Extraction Optimization of Pedunculoside in Ilex rotunda Thunb.by Orthogonal Test[J]. China Pharmacist, 2012, 15(6): 805-807 | |
| Authors: | Wang Haisen Li Chaosheng Wang Jing Li Zhe Yu Qiangqiang Hua Shan Gu Yu |
| Affiliation: | Jinlin Agricultural University, Changchun 130118, China;;Jilin Academy of Chinese Medical Sciences;;Jilin Academy of Chinese Medical Sciences;;Yanbian University;Jinlin Agricultural University, Changchun 130118, China;;Yanbian University;Jinlin Agricultural University, Changchun 130118, China; |
| Abstract: | Objective: To optimize the extraction technology of pedunculoside in Ilex rotunda Thunb.Method: Alcohol with different concentrations as the solvent,the amount of the solvent,the extraction time and the extraction frequency were optimized by orthogonal test with the extraction rate of pedunculoside as the index.Pedunculoside content was determined by HPLC.Result: The optimum extraction conditions were as follows:6 times of 70% alcohol as the solvent,reflux extraction 3 times with 1h each.Conclusion: The procedure is efficient with lower energy and shortened time.It is suitable for the industrial production. |
| Keywords: | Orthogonal test Ilex rotunda Thunb. Pedunculoside Extraction technology |
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