肺泡表面活性物质对肺挫伤后肺泡巨噬细胞功能的影响

目的 观察研究外源性猪肺表面活性物质(PPS)对肺挫伤大鼠肺泡巨噬细胞(AM)的吞噬功能和分泌功能的影响,探讨补充PPS对肺挫伤大鼠的治疗作用及其机制.方法 采用贴壁培养的方法,分离肺挫伤大鼠肺泡灌洗液中的AM,将分离的AM于普通培养液、含PPS(100μg/ml或200 μg/ml)的培养液、含LPS(20 μg/ml)的培养液或含LPS(20 μg/ml)+PPS(100 μg/ml或200 μg/ml)的培养液中培养2h后,采用真菌吞噬法测定各组细胞的吞噬功能；采用RTPCR方法测定各组细胞中TNF-α mRNA含量.结果 与对照相比,PPS单独作用对AM的吞噬功能和TNF-α mRNA含量无明显影响.经LPS刺激后AM的吞噬功能增强,TNF-α mRNA含量明显增高.PPS对LPS导致的吞噬功能增强无明显作用,但是能明显降低TNF-α mRNA含量.结论 PPS对AM的吞噬功能影响不大,但可以抑制TNF-α mRNA表达.

Effects of pulmonary surfactant on alveolar macrophage function after pulmonary contusion

Objective To observe the effects of porcine pulmonary surfactant(PPS) on the function of pulmonary alveolar macrophages(AMs) in vitro,so as to explore the mechanism by which PPS treating pulmonary contusion in rats. Methods AMs were separated by adherent culture from bronchoalveolar lavage fluid of rats with pulmonary contusion.The AMs were then cultured with media containing PPS(100 μg/ml or 200 μg/ml),LPS(20 μg/ml)+PPS(100 μg/ml or 200 μg/ml),or LPS(20 μg/ml) alone for 2 h.Then the phagocytic function of AMs in each group was examined with fungus.TNF-α mRNA was determined by RT-PCR in AMs of each group.AMs untreated with PPS and LPS were taken as blank control. Results The phagocytic function of the AMs and the expression of TNF-α mRNA were not significantly affected by PPS alone compared with the control group.LPS stimulation increased the phagocytic function of AMs and the TNF-α mRNA expression in AMs.PPS showed no significant effect on LPS-induced increase of phagocytic function of AMs,but it could greatly inhibit LPS-induced TNF-α mRNA increase. Conclusion PPS has no noticeable effect on the phagocytic function of the AMs,but it can inhibit TNF-α mRNA expression induced by LPS in AMs.