氘代内标液质串联法测定血浆中沙丁胺醇的浓度

目的:建立液相-质谱串联法测定人血浆中的沙丁胺醇含量.方法:血浆离心后过玻璃纤维滤膜,经酶解加入氘代沙丁胺醇内标溶液,用C18小柱净化后以3%的氨水甲醇溶液对Oasis MCX小柱进行洗脱,吹干,以0.1%甲酸水溶液-甲醇溶液(95∶5)溶解残余物,用液相质谱串联法测定,以0.1%甲酸乙腈溶液和0.1%甲酸溶液为流动相梯度洗脱.结果:沙丁胺醇在0.25～10.00 μg·kg-1线性关系良好,检出限0.1μg·kg-1.结论:本法灵敏准确、重现性与特异性强、干扰少,可用于人体内沙丁胺醇药动学与生物利用度研究.

Determination of Salbutamol in Human Plasma by LC/MS/MS with Deuterated Internal Standard

Objective: To study the determination of salbutamol in human plasma by LC/MS/MS.Method: The deuterated salbutamol internal standard was added to the enzymatic hydrolyzed plasma samples after filtrated by glass fiber,then purified by C18 cartridges and eluted by a solvent composed of 3% ammonia methanol through Oasis MCX cartridges.The eluent was dried and the remains were dissolved with 0.1% formic acid solution-95% methanol followed by LC/MS/MS determination with gradient elution by the mobile phase composed by 0.1% acetonitrile formate solution and 0.1% formic acid solution.Result: Salbutamol in human plasma samples was in good linear within the concentrations range of 0.25-10.00μg·kg-1 with the detection limit of 0.1μg·kg-1.Conclusion: The method is sensitive,accurate,repeatable and specific with little interference,and suitable for the inspection of pharmacokinetics and bioavailability of salbutamol in vivo.