肠泻停胶囊对实验性结肠炎大鼠的影响

目的:探讨肠泻停胶囊对三硝基苯磺酸(TNBS)诱导大鼠实验性结肠炎的影响.方法:120只SD大鼠随机分为6组,每组20只:正常组、模型组、阳性对照组、肠泻停胶囊高、中、低组.除正常对照组未行造模外,其余五组大鼠均采用TNBS造模.肠泻停胶囊高、中、低组分别灌胃给药(1.80,0.90,0.45 g /kg体质量)、阳性对照组灌胃给予地塞米松剂量(0.2 mg/kg体质量)、其余组灌胃给予0.5%羧甲基纤维素钠溶液连续3 周、4周;观察肠泻停胶囊对大鼠腹泻率、死亡率、血白细胞计数、淋巴细胞百分率、脾脏及胸腺重量、组织形态学评分、组织MPO活性的影响.结果:经肠泻停胶囊干预后各剂量组动物腹泻明显缓解,腹泻率降低,动物死亡率降低,外周血WBC、LYM值及组织MPO值降低,剖检可见结肠组织溃疡面积明显缩小,水肿缓解,坏死减轻,未见肠壁增厚.结论:肠泻停胶囊连续给药,对实验性结肠炎治疗作用显著.

Effect of Changxieting Capsules on Experimental Colitis in Rats

Objective: To explore the effect of Changxieting capsules(CXT) on trinitrobenzene sulfonic acid(TNBS)-induced experimental colitis in rats.Method: 120 rats were randomly divided into the following six groups(20 in each group),the normal group,mode1 group,contro1 group,CXT capsule group with high,medium and low dose,respectively.The experimental colitis in rats were induced by TNBS in the groups except those in the normal group.The rats in the CXT capsule group with high,medium and low dose were given CXT capsules(1.80,0.90 and 0.45 g/kg body weight),those in the contro1 group were given dexamethasone(0.2 mg /kg body weight) and those in the normal group and mode1 group were given 0.5% sodium carboxymethyl cellulose through intragastric administration for 3w and 4w.The effects of CXT capsules on diarrhea percent,mortality,white cell count,lymphocyte percent,spleen and thymus weight,histomorphology score and tissue MPO activity were observed.Result: The diarrhea percent,mortality,white cell count,lymphocyte percent and tissue MPO activity were obviously improved in the CXT capsule groups compared with those in the mode1 group.Conclusion: CXT capsules show notable efficacy on the experimental colitis in rats.