| HPLC法测定参芪生肌颗粒中阿魏酸的含量 |
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| 引用本文： | 俞永梅,张平,牟娜.HPLC法测定参芪生肌颗粒中阿魏酸的含量[J].中国药师,2012,15(6):826-828. |
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| 作者姓名： | 俞永梅 张平 牟娜 |
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| 作者单位： | 上海市松江食品药品检验所,上海,201600 |
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| 摘 要： | 目的:采用HPLC法测定参芪生肌颗粒中阿魏酸的含量.方法:采用70%甲醇超声提取,HPLC法测定.Kromasil C18柱(150 mm×4.6 mm,5 μm)分离;柱温30℃;流动相为甲醇-0.1%磷酸溶液(30:70);流速为1.0 ml·min-1;检测波长为320 nm.结果:阿魏酸的线性范围为1.578～9.468 μg(r=0.999 8);平均回收率为99.34%,RSD为1.2%(n =9).结论:方法简便、快速准确、灵敏度高,重复性好.
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| 关 键 词： | 阿魏酸 高效液相色谱法 参芪生肌颗粒 含量测定 |
| 收稿时间： | 2011/12/28 0:00:00 |
| 修稿时间： | 2012/3/15 0:00:00 |
Determination of Ferulic Acid in Shenqi Shengji Granules by HPLC |
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| Yu Yongmei,Zhang Ping and Mou Na.Determination of Ferulic Acid in Shenqi Shengji Granules by HPLC[J].China Pharmacist,2012,15(6):826-828. |
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| Authors: | Yu Yongmei Zhang Ping and Mou Na |
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| Institution: | (Shanghai Songjiang Institute for Food and Drug Control,Shanghai 201600,China) |
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| Abstract: | Objective: To determine ferulic acid in Shenqi Shengji granules by HPLC.Method: The samples were extracted by ultrasonic with 70% methanol,and an ion pair HPLC method was applied to determine the content of ferulic acid.The column of Kromasil C18(150 mm×4.6 mm,5 μm) with temperature of 30℃ was used to separate the target components,the mobile phase was composed of methanol-0.1% phosphate solution(30∶ 70),the flow rate was 1.0 ml·min-1 and the detection wavelength was at 320 nm.Result: The linear range was 1.578-9.468 μg(r=0.999 8),the average recovery was 99.34% with RSD of 1.2%(n=9).Conclusion: The method is simple,sensitive and reliable. |
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| Keywords: | Ferulic acid HPLC Shenqi Shengji granules Content determination |
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