广西东兰县壮族长寿老人HLA-DRB1基因的多态性研究

目的：探讨人类白细胞抗原-DRB1（HLA-DRB1）基因与长寿的相关性。方法：应用聚合酶链式反应-序列特异引物（PCR—SSP）对东兰县150名90岁以上长寿老人（90～105岁）和143名当地健康、无血缘关系、随机抽样的成年人（20～76岁）进行HLA-DRB1基因分型并进行相应等位基因频率的比较。结果：长寿组HLA—DRB1＊1201-1202等位基因频率（10．56％）明显高于对照组（5．39％）（P=0．0239，OR=1．9067）；其余各相应等位基因频率之间的比较差异无统计学意义（均P〉0．05）。另外，在HLA—DRB1座位各等位基因中，长寿组和对照组均以HLA-DRB1＊1501等位基因的频率最高，分别为29．76％、24．74％；其次为HLA—DRB1＊1601-1602等位基因，分别为17．94％、20．23％。结论：在东兰县壮族人群中，HLA—DRB1＊1201—1202与长寿高度关联，可能通过某种未知的机制影响人类的衰老及寿命。

A STUDY ON DNA POLYMORPHISM OF THE HLA-DRB1 LOCUS OF DONGLAN ZHUANG NATIONALITY IN GUANGXI PROVINCE AND ITS ASSCOCIATION WITH HUMAN LONGEVITY

Objective:To investigate a possible association between HLA-DRB1 and longevity.Methods:we genotyped the HLA-DRB1 locus with Polymerase Chain Reaction-sequence specific primer(PCR-SSP) method for 150 longevous people(90-105years) and 143 healthy,unrelated,randomly selected controls(20-76years) who live in the same area.All individuals tested are all Donglan Zhuang nationality which can be traced back at least 8 generations.Result:The frequency of HLA-DRB1*1201-1202 allele is significantly high in longevous group,as compared to that of the control group(10.56%,5.39% respectively,P=0.023 9,OR=1.906 7).No changes were found in other alleles between the two groups(P>0.05).The most common allele on HLA-DRB1 locus is HLA-DRB1*1501(29.76% in longevous group,24.74% in controls),then follows HLA-DRB1*1501(17.94% and 20.23% in longevous group and control group respectively).Conclusion:Our data suggested that HLA-DRB1*1201-02 might have involved in the mechanism of human longevity in Donglan Zhuang population,which is worthy of further investigation.