Serum IgA and IgG antibodies to α-gliadin: Comparison between two ELISA methods |
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| Authors: | Sandra Perticarari Gianni Presani Marina Trevisan Adriano Savoini Sabina Cauci |
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| Affiliation: | (1) Laboratorio di Analisi Biochimico-Cliniche, Istituto per l’Infanzia, Via dell’Istria 65/1, 34100 Trieste, Italia;(2) Servizio di Gastroenterologia, Istituto per l’Infanzia, Trieste;(3) Dipartimento di Biologia, Università degli Studi di Trieste, Italia;(4) Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università degli Studi di Trieste, Italia |
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| Abstract: | Summary We have developed two ELISA methods, i.e., enzyme immunoassay (EIA) and fluorescence immunoassay (FIA), for the semiquantitative detection of specific IgA and IgG antibodies directed against α-gliadin. The tests differ only for the enzyme substrate and, when optimized, could be used in large routine screening of celiac disease. Several serum samples from patients with celiac disease and gastrointestinal disorders as well as from control subjects were tested. Both methods gave good correlation with clinical data, were easily performed and had same specificity features, while FIA proved to be more sensitive. |
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| Keywords: | α -gliadin Celiac disease ELISA technique IgA and IgG antibodies |
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