B72.3第二代单克隆抗体的研制和免疫组化分析

以Ｂ７２．３单抗的对应肿瘤相关糖蛋白抗原７２（ＴＡＧ－７２）为免疫源，采用细胞融合技术制备出了Ｂ７２．３第二代单克隆抗体７２－４５和７２－１４２。上述单抗的免疫组化分析结果表明，除同胎儿和成人皮脂腺、汗腺和小肠上皮细胞呈一定程度的反应外，同其它正常组织器官均呈阴性反应。相反，单抗７２－４５在大肠癌的阳性率为１００％，肺腺癌为８０％。单抗７２－１４２在大肠癌阳性率为４０．５％，肺腺癌为７９．５％。结果提示上述单抗对恶性肿瘤的诊断和治疗具有一定的潜在应用价值。

Generation and Immunohistochemical Analysis of MAb B72.3 Secondary Generation Antibodies

Ab B7 2.3 secondary antibodies 72-45 and 72-142 were generated by using the TAG-72 antigen asimmunogen and the technique of hybridoma.Immunohistochemical analysis have shown that the twoantibodies do not react with majority of fetal and adult human tissues except sebaceous gland,sweatgland and the epithelial cells of small intestine,Reversely,100%(20/20)are positive in colon cancer and80%(24/30) in lung adenocarcinoma with MAb 72-45, 40.5%positive in colon cancer and 79.5%(27/34)in lung adenocarcinoma with MAb 72-142.The above results suggest that the antibodies may bevaluable in tumor targetting therapy and diagnosis.