| 人类白细胞介素2基因重组子cDNA功能片段的碱基序列分析 |
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| 引用本文： | 任常山,陆世龙.人类白细胞介素2基因重组子cDNA功能片段的碱基序列分析[J].中国医科大学学报,1994,23(1):1-3. |
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| 作者姓名： | 任常山 陆世龙 |
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| 摘 要： | 应用Ｓａｎｇｅｒ的双脱氧链终止法，对本室构建的人类白细胞介素２基因重组子ｐＲＥＴ－９０的４４０ｂｐｃＤＮＡ功能片段（ＮｃｏＩ克隆点的ＨｇｉＡＩ－ＤｒａＩ片段）进行ＤＮＡ碱基序列分析。结果表明：白细胞介素２ｃＤＮＡ功能片段按照正确的方向和翻译顺序连接在载体ｐＥＴ３ｄ的ＮｃｏＩ－ＢａｍＨＩ切点之间。
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| 关 键 词： | 人类白细胞介素2 基因工程 碱基序列分析 |
Nucleotide Sequence Determination of cDNA Fragment of Human Interleukin 2 Gene |
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| Ren
Chanshan,Lu Shilong,Chen Hong,et al..Nucleotide Sequence Determination of cDNA Fragment of Human Interleukin 2 Gene[J].Journal of China Medical University,1994,23(1):1-3. |
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| Authors: | Ren
Chanshan Lu Shilong Chen Hong |
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| Institution: | Cancer Research Institute |
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| Abstract: | y using Sanger's
dideoxy methods of DNA sequencing,44 0bp cDNA fragment(HgiA I- Dra Ifragment in Nco I cloning
site)of recombinant human interleukin-2 plasmid pRET-90 was analysed.The result showed that
the cDNA fragment of IL-2 was linked in the proper orientation and readingframe of Nco I -BamH I
fragment of pET3d. |
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| Keywords: | human interleukin-2 gene engineering DNA sequencing |
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