光感基因调控技术对大鼠骶上脊髓损伤后膀胱尿动力学和肌电图的影响

目的观察光感基因调控技术对大鼠骶上脊髓损伤所致神经源性膀胱功能的影响。方法 50只大鼠经尿流动力学检查无异常后进行随机分组,并采用T10脊髓完全横断建立脊髓损伤动物模型,分为假手术对照组、脊髓损伤无蓝光刺激组和脊髓损伤蓝光刺激组。2周后进行膀胱尿动力学、肌电图测定。结果脊髓损伤蓝光刺激组大鼠膀胱逼尿肌肌条舒缩曲线大部分可见规律性变化,波形均匀一致;同时膀胱最大容量增加,内压降低,顺应性升高;而脊髓损伤无蓝光刺激组无上述变化。结论光感基因可以调节骶上脊髓完全性损伤后膀胱逼尿肌的收缩功能,对神经源性膀胱功能恢复有重要意义。

The effect of light gene regulation on the bladder urinary and electromyography of neurogen-ic bladder function recovery by the upper-sacral spinal cord injury in the rats

Abtract:Objective To observe the effect of light gene regulation on neurogenic bladder function recovery of the up-per-sacral spinal cord injury in rats. Methods Fifty rats which had no abnormalities after urine flow dynamic test were randomized,and the 10th thoracic spinal cord complete transection animal model was set up,which were divided into control group,no blue light stimulation of spinal cord injury group and blue light stimulation of spinal cord injury group. The bladder urinary and electromyography were assessed after 2 weeks. Results The contraction curve of the bladder detrusor muscle strips in rats was regular in the electromyography while bladder pressure was remarkably de-creased and the maximum bladder capacity and bladder compliance were increased,which was not found in no blue light stimulation of spinal cord injury group. Conclusions Light gene can regulate the neurogenic bladder dysfunc-tion of upper-sacral spinal cord injury,which has an important significance in the neurogenic bladder function recover-y.