白藜芦醇对七氟醚诱导神经细胞损伤的影响

目的探讨白藜芦醇(resveratrol,RES)对七氟醚诱导的离体神经细胞损伤的影响及其机制。方法取SD孕鼠的胎鼠海马组织,分离培养原代神经细胞,然后将细胞随机分为四组:对照组(C组),正常培养的原代神经细胞;3%七氟醚组(S组),3%七氟醚混合气体处理的细胞;3%七氟醚+RES组(SR组),用RES处理细胞6h后,再用3%七氟醚混合气体处理的细胞;RES组(R组),RES处理的细胞。流式细胞仪检测神经细胞的凋亡;CCK-8试剂盒检测神经细胞的增殖;Western blot检测Caspase-3、Bax、Bcl-2和Bace-1的表达水平;ELISA检测APP和Aβ的表达水平。结果 S组的细胞凋亡率、Caspase-3、Bax、Bace-1、APP及Aβ的表达明显高于C组(P0.05);细胞增殖能力和Bcl-2的表达明显低于C组(P0.05)。SR组的细胞凋亡率、Caspase-3、Bax、Bace-1、APP及Aβ的表达明显低于S组(P0.05);细胞增殖能力、Bcl-2的表达明显高于S组(P0.05)。结论白藜芦醇通过减少细胞凋亡,促进细胞增殖以缓解七氟醚诱导的离体神经细胞损伤,这可能与Aβ的表达下调,从而降低神经毒性有关。

Effect of resveratrol on sevoflurane-induced nerve cell injury

Objective To investigate the effect of resveratrol (RES)on sevoflurane-induced nerve cell injury and its mechanism.Methods Fetal hippocampal tissues were taken from SD pregnant rats and primary nerve cells were isolated and cultured,then the cells were randomly divided into four groups:group control (group C),normal primary nerve cells;group 3% sevoflurane (group S),cells treated with 3% sevoflurane gas;group 3% sevoflurane+RES (group SR),cells treated with RES for 6 h,and then treated with 3% sevoflurane gas;group RES (group R),cells treated with RES.Flow cytometry assay was used to detect the apoptosis of nerve cell;CCK-8 Kit was used to detect cell proliferation;Western blot was used to detect the expression level of Caspase-3,Bax,Bcl-2 and Bace-1;ELISA method was used to detect the protein level of amyloid precursor protein (APP)andβ-amyloid peptide (Aβ).Results Compared with the group C,cell apoptosis was increased (P <0.05),cell proliferation was decreased (P <0.05),the expression levels of Caspase-3 and Bax were up-regulated (P <0.05),the expression level of Bcl-2 was down-regulated (P <0.05), and the protein levels of Bace-1,APP and Aβwere significantly decreased by 3% sevoflurane treat-ment (P <0.05),which were all significantly reversed by RES treatment.Compared with group S, cell apoptosis was decreased (P <0.05),cell proliferation was increased (P <0.05),the expression levels of Caspase-3 and Bax were down-regulated (P <0.05),the expression level of Bcl-2 was up-regulated (P <0.05),and the protein levels of Bace-1,APP and Aβwere increased in group SR (P <0.05).Conclusion Resveratrol alleviated sevoflurane-induced nerve cell injury in vitro by reducing cell apoptosis and promoting cell proliferation,which may be related with the reduction of Aβexpression and the reduced Aβ-induced neurotoxicity.