慢性牙周炎患者基础治疗前后龈沟液中瘦素水平变化的研究

目的：检测慢性牙周炎患者牙周基础治疗前后龈沟液中瘦素水平的变化。方法：选择轻、中、重度牙周炎患者共3组，每组11人，基础治疗前后收集龈沟液，采用酶联免疫法（Enzyme-Linked ImmunoSorbent Assay，ELISA）检测瘦素含量。结果：3组患者牙周基础治疗后龈沟液中瘦素水平均明显高于治疗前（P＜0.01）；瘦素与轻度牙周炎组治疗前的出血指数正相关（r=0.675）（P＜0.05），与重度牙周炎组治疗前的探诊深度负相关（r=-0.799）（P＜0.01）；重度牙周炎组治疗后比治疗前的探诊深度减小（P＜0.01），附着丧失减小（P＜0.05）。结论：龈沟液中瘦素含量变化与牙周炎的基础治疗密切相关，可以通过测定瘦素水平评估牙周炎治疗的临床疗效。

The change of Leptin in GCF before and after initial therapy of Chronic periodontitis

Objective:To evaluate and compare gingival crevicular fluid (GCF)leptin levels before and after initial therapy of periodontitis.Then to obtain an insight into leptin’s possible role in periodontal therapy. Method:A total of 33 subjects, they were categorized into 3 groups of 11 subjects each,based on their periodontal status,as follows:Group Ⅰ(mild periodontitis);GroupⅡ (moderate periodontitis);GroupⅢ (severe periodontitis). GCF samples were collected before and after periodontal initial therapy,and samples were analyzed for leptin using ELISA. Result:In all the three groups,the concentration of leptin after the treatment was higher than before ( P<0.01). Leptin was related to bleeding index before the therapy of mild periodontitis (r = 0.675) (P<0.05), and had a negative correlation with probing depth before the therapy of severe periodontitis(r=0.799) (P< 0.01);The probing depth of treated severe periodontitis was shallower than that before the therapy (P<0.01),the attachment loss of treated severe periodontitis was decreased.(P<0.05). Conclusion:Leptin levels in GCF was closely related to the therapy of periodontitis, and the leptin levels may be used as an objective indicator to evaluate the therapy of periodontitis.