| DGGE分析中间普雷沃菌在原发性根尖周感染中的基因多态性 |
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| 引用本文： | 邹岩,漆正楠,黄正蔚,何智妍,朱彩莲,唐子圣. DGGE分析中间普雷沃菌在原发性根尖周感染中的基因多态性[J]. 临床口腔医学杂志, 2016, 0(9): 523-526. DOI: 10.3969/j.issn.1003-1634.2016.09.004 |
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| 作者姓名： | 邹岩 漆正楠 黄正蔚 何智妍 朱彩莲 唐子圣 |
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| 作者单位： | 1. 上海交通大学医学院附属第九人民医院牙体牙髓科·上海市口腔医学重点实验室 上海 200011;2. 上海市口腔病防治院口腔内科 上海 200001;3. 上海交通大学医学院附属第九人民医院·口腔医学研究所 上海 200011 |
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| 基金项目： | 上海市自然科学基金(13ZR1423900)；上海交通大学“医工(理)交叉研究”基金资助项目(YG2013MS58) |
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| 摘 要： | 目的：检测中间普雷沃菌在原发性根尖周感染中的基因多态性。方法：收集17例原发性根尖周感染根管内的细菌学样本，针对其16 S rDNA V3?V6区段进行PCR?DGGE，割胶测序，序列比对。结果：中间普雷沃菌在原发性根尖周感染中的检出率为47?06％。 DGGE图谱中显示14条不同位置的条带，割胶测序后发现有3个不同的基因型。结论：通过PCR?DGGE证实了中间普雷沃菌在原发性根尖周感染中存在基因多态性。
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| 关 键 词： | 原发性根尖周炎 中间普雷沃菌 基因多态性 变性梯度凝胶电泳 |
Genotypic analyses of Prevotella intermedia in primary endodontic infections by DGGE |
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| Abstract: | Objective: To analyze the genetic diversity of Prevotella intermedia in primary endodontic infections?Methods:Seventeen microbial samples were taken from the root canals of teeth with primary endodontic infec?tions?DNA extracted from the samples was amplified by nested PCR to detect P?intermedia?It was conducted with P?intermedia ?specific primers based on 16 S rDNA V3?V6 region,and several DGGE bands were excised for further sequen?cing?Results:The prevalence of P?intermediain primary endodontic infections was 47?06%?There were 14 DGGE bands present at different position?It was founded 3 genetic according to sequencing and blasting?Conclusion:Genetic diversity of P?intermedia was presented by PCR?DGGE. |
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| Keywords: | Primary endodontic infections Prevotella intermedia Genepolymorphism Denatured gradient gel elec-trophoresis |
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