兔自体松质骨颗粒修复关节软骨损伤

目的研究兔自体松质骨颗粒在膝关节软骨损伤处移植后能够诱导软骨组织生成、促进关节软骨损伤修复的现象。方法 12只新西兰大白兔麻醉后在兔的右侧膝关节股骨远端内、外侧髁负重区用电钻分别造成直径、深度均为3 mm的骨软骨缺损创面,取同侧髂骨松质骨,制成直径约为1 mm松质骨颗粒植入股骨内侧髁软骨缺损处,作为实验组,外侧髁软骨缺损不做处理作为对照组。术后12周进行膝关节大体观察、病理切片染色,评估关节软骨损伤的修复情况。结果兔膝关节实验组软骨缺损处被新生软骨填充,软骨面光滑,组织切片染色显示有关节软骨形成。对照组缺损创面仍然凹陷,仅在缺损边缘有少量软骨生长。结论兔自体松质骨颗粒在膝关节软骨损伤处能够诱导软骨生成,促进关节软骨的修复,是一种良好的关节软骨损伤修复方法。

Repair of rabbits articular cartilage defect by autogenous cancellous bone particles

Objective To study the phenomenon that transplantation of the autogenous cancellous bone particles in cartilage defect of the rabbit′s knee joint can induce generation of cartilage tissue and promote the repair of articular cartilage defect. Methods After anaesthesia, 12 New Zealand white rabbit were made same cartilage defect in femo-ral medial condyle and lateral condyle of right knee, the depth and diameter were both 3 mm. As the experimental group, the cartilage defect in femoral medial condyle was repaired by autologous cancellou bone particle incise the rabbit′s iliac bone ( diameter of 1 mm ) the cartilage defect in femoral lateral condyle was not repaired as control group. To assess the therapeutic effect of the two different therapy methods, the gross specimen, the pathological stai-ning and the repair effect of the cartilage defect were observed at 12 weeks after surgery. Results The cartilage de-fect that was implanted by autologous cancellous bone particle had been repaired by neocartilage, the surface was smooth, the section staining shown cartilago articularis was generated. But in femoral lateral condyle that was not re-paired only little neocartilage appear at the edge of the defect, the defect was seen clearly. Conclusions The trans-plantation of the autogenous cancellous bone particles in cartilage defect of the rabbit knee joint can induce generation of cartilage tissue and promote the repair of articular cartilage defect. It is a good method for repair of articular carti-lage injury.