RP-HPLC同时测定藏药波棱瓜子中7个活性木脂素成分的含量

 目的建立一种RP-HPLC同时测定藏药波棱瓜子中7个活性木脂素成分(ent-isolariciresinol,dehydrodiconiferyl alcohol,herpet-rione,herpetin,herpetetrone,herpetotriol,herpetal)的含量。方法采用Pinnacle DB C₁₈(4.6 mm×250 mm,5μm,RESTEK)色谱柱,以乙腈-0.1%磷酸为流动相梯度洗脱,检测波长为240 nm,流速为1.0 mL·min^-1。结果Ent-isolariciresinol,dehydrodiconiferyl alcohol,herpetrione,herpetin,herpetetrone,herpetotriol和herpetal的线性范围分别为:1.29～24.80 mg·L^-1(r=0.978 9),6.77～130.20 mg·L^-1(r=0.100 26),10.41～200.20 mg·L^-1(r=0.980 2),4.45～85.50(r=0.986 1),2.91～56.10 mg·L^-1(r=0.982 8),5.80～111.60mg·L^-1(r=0.100 78)和6.99～134.40 mg·L^-1(r=0.986 6)。平均回收率均在95.19%～102.64%内(RSDs<1.52%)。结论本法准确可靠,灵敏度高,重现性好,结果表明,该分析方法适用于波棱瓜子药材的质量控制。

Simultaneous Determination of 7 Bioactive Lignans Compounds in Herpetospermum caudigerum by RPHPLC

OBJECTIVE To establish a method for the determination of 7 bioactive lignan compounds in seeds of Herpetospermum caudigerum,namely ent-isolariciresinol,dehydrodiconiferyl alcohol,herpetrione,herpetin,herpetetrone,herpetotriol,herpetal.METHODS The HPLC assay was performed on a RESTEK Pinnacle DB C₁₈ column with gradient elution.The mobile phase consisted of acetonitrile and 0.1% phosphoric acid at a flow rate of 1.0 mL·min^-1.The detection wavelength was 240 nm.RESULTS The linearity was obtained over the ranges of 1.29-24.80 mg·L^-1(r=0.978 9) for ent-isolariciresinol,6.77-130.20 mg·L^-1(r=0.100 26) for dehydrodiconiferyl alcohol,10.41-200.20 mg·L^-1(r=0.980 2) for herpetrione,45-85.50 mg·L^-1(r=0.986 1) for herpetin,2.91-56.10 mg·L^-1(r=0.982 8) for herpetetrone,5.80-111.60 mg·L^-1(r=0.100 78) for herpetotriol and 6.99-134.40 mg·L^-1(r=0.986 6) for herpetal,respectively.The recovery of the assay was in the range of 95.19%-102.64% with RSDs less than 1.52%.CONCLUSION The method is simple,sensitive and reproducible,and the results indicated that the developed assay could be considered as a suitable quality control method for Herpetospermum caudigerum.