Etude du couplage chromatographie d'exclusion-diffusion de la lumière, 1. Dispositif expérimental et étude de polystyrènes «références»

The possibilities of coupling size exclusion chromatography with light scattering (GPC-DDL) are studied. The continuous light scattering detector is a modified (laser He-Ne excitation) classical light scattering apparatus Fica 42000, equipped with a flow cell of low capacity (120μl). With this cell, a detection of the scattered light is obtained only at an angle of 90°. The light scattering detector is coupled with a GPC apparatus (packed with 10 μm gel particles). The precision, reproducibility and limitation to obtain molecular weight distribution curves of “standard” linear polystyrenes are studied.