| Abstract: | Exposure of isolated frog rod outer segments (ROS) to ferrous sulfate in a Krebsbicarbonate medium causes a time-dependent disruption of the membrane organization of the discs. Ferrous sulfate also causes ROS swelling and aggregation. Addition of taurine (5–20 mM) and zinc sulfate (250 μM) to the incubation medium markedly protected ROS from the disrupting effect of ferrous sulfate. Of other amino acids tested, only β-alanine had a protective effect on ROS structure. Ferrous sulfate caused an increase in lipid peroxidation, measured by malonaldehyde formation. The protective effect of taurine and zinc is not accompanied by a reduction of lipid peroxidation. Water accumulation occurs as a consequence of the peroxidative action of ferrous sulfate, and this effect was counteracted by taurine and zinc. Ferrous sulfate did not cause damage to ROS structure when incubation was carried out in sucrose-HEPES. Sodium, chloride, and bicarbonate ions caused ferrous sulfate to disrupt ROS structure. It is concluded that taurine and zinc protect ROS membranes from ion and/or water entry occurring as a consequence of membrane lipid peroxidation. |
