淋球菌毒力岛分类及sac-4基因对淋球菌血清抗性作用的研究

目的 研究淋球菌不同基因型别的毒力岛类型及其sac-4基因与淋球菌血清抗性作用的关系.方法 运用NG-mast和正常人血清(NHS)的抗性比较试验,对46株淋球菌从分子水平上进行基因分型和毒力岛分类,并在此基础上探讨毒力岛sac-4基因与淋球菌NHS抗性之间的关系.结果 46株野生株中,GGI阳性菌株占80.43%,可分为含有traG和altA;含带sac-4t基因的traG和altA;只含有traG一部分保守序列的三种毒力岛类型.血清抗性实验表明9株sac-4阳性的淋球菌野生株均不具有血清抗性,而sac-4阴性的2号和11号菌株却产生了部分血清抗性和完全抗性.结论 不同毒力岛类型有着不同的毒力特点和基因型别,淋球菌血清抗性作用与毒力岛sac-4基因并无明显相关性.

Study on the classification of gonococcal island of different genotypes and effects of sac-4 gene on serum resistance of Neisseria gonorrhoeae

Objective Using molecular methods to study the relationship between genotypes and serum resmtance of Neisseria gonorrhoeae in Wuhan area.Methods NG-mast and serum bactericidal assays at the molecular level were used to differentiate the 46 strains which were isolated from the outpatients of sexually transmitted disease clinics and the relationship between different genotypes while phenotypes was also studied.Results 80.43% of the 46 strains contained the island and we were able to define three dillerent combinations of genes in the isolates.Results from serum bactericidal assays showed that all 9 sac-4 stralns did not provide any serum resistance.Conclusion Different isolates carried clifferent gonococcal genetm islands(pathogenicity island)and certain phenotypes.There were no sobious relationship between sac-4 gene and serum resistance of Neisseria gonorrhoeae.