曲尼司特对动脉损伤后血管外膜重塑及细胞增殖的作用

 【目的】血管损伤后外膜反应是负性血管重塑的重要机制。本文通过观察曲尼司特对损伤血管外膜反应和细胞增殖的作用,探讨其对血管重塑作用的可能机制。【方法】70只新西兰兔随机分为2组:曲尼司特组和安慰剂组,采用病理学和免疫组化技术,观察曲尼司特对兔腹主动脉损伤后外膜重塑、外膜细胞增殖和细胞表型的作用。【结果】血管损伤后28d,曲尼司特组外膜厚度降低29.4%。血管损伤后14d和28d,曲尼司特组外膜面积分别降低22.6%和20.6%。血管损伤后7d和14d,曲尼司特组外膜细胞密度分别降低22.7%和38.9%。血管损伤后3d和7d,曲尼司特组外膜PCNA阳性细胞百分比分别降低31.4%和29.6%。【结论】曲尼司特在血管损伤后早期显著抑制外膜细胞增殖,通过减少外膜的细胞容积,明显降低外膜厚度和面积,抑制血管重塑。

Effects of Tranilast on Remodeling and Cell Proliferation of Adventitia after Vascular Injury

Objective] As the important mechanism of negative vascular remodeling, the adventitia reaction after arterial injury has been investigated for several years. We attempt to comprehend the potential mechanism of tranilast, an anti-allergic drug, on vascular remodeling by observing the change of adventitia reaction and cell proliferation after vascular injury. Methods] Seventy New Zealand rabbits were randomly divided into two groups in this study, as tranilast group and placebo group. After abdominal artery injury, the effects of tranilast on adventitial remodeling, cell proliferation and cell phenotype shift were observed by pathological and immunohistochemistry technique. Results] Comparing to that in control group, tranilast decreased the thickness of adventitia by 29.4% at the twenty-eighth day after vascular injury, and the area of adventitia by 22.6% and 20.6% at the fourteenth day and the twenty-eighth day after vascular injury respectively. In tranilast group, the cell density in adventitia was reduced by 22.7% and 38.9% at the seventh day and the fourteenth day after vascular injury respectively, and the percentage of PCNA-positive cell in adventitia by 31.4% and 29.6% at the third day and at the seventh day after vascular injury respectively. Conclusion] Tranilast inhabit the injury reaction and cell hyperplasia in adventitia after vascular injury, which act as the key of negative vascular remodeling in athesclerosis and restenosis.