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| In Vitro Cytotoxicity of Polyphosphoester as a Novel Injectable Alveolar Replacement Material | |
| 作者姓名： | 张智星 毛靖 冯祥礼 肖建中 邱进俊 |
| 作者单位： | Department of Orthodontics，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Nursing，Tongji Medical College，Huazhong University of Science and Technology，College of Materials Science and Engineering，Huazhong University of Science and Technology，College of Chemistry，Huazhong University of Science and Technology |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | The aim of this study was to investigate the in vitro cytotoxicity of polyphosphoester polymer used as a novel injectable alveolar bone substitutes for controlled delivery of tetracycline. Cell culture medium was exposed to the polymer （0.01-10 mg/mL） for 24 h. The L-929 mouse fibro- blasts were then exposed to the treated cell culture medium for 24 h. Finally, cell viability and growth were assessed by using MTT assay and Alamar Blue assay. No significant cytotoxicity of the polyphosphoester against L-929 mouse fibroblasts was observed at a concentration up to 10 mg/mL （P〉0.05）. The two evaluation methods showed no significant differences （P〉0.05）. This study suggests that polyphosphoester does not demonstrate any significant toxic effects to cells in vitro and has the potential to be used both as a medical device and as scaffolds in tissue engineering applications. |
| 关 键 词： | 体外细胞毒性 聚磷酸酯 可注射 鼠成纤维细胞 材料 肺泡 细胞培养基 细胞培养液 |
<Emphasis Type="Italic">In vitro</Emphasis> cytotoxicity of polyphosphoester as a novel injectable alveolar replacement material |
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| Zhixing?Zhang,Jing?Mao,Xiangli?Feng,Jianzhong?Xiao,Jinjun?Qiu.In Vitro Cytotoxicity of Polyphosphoester as a Novel Injectable Alveolar Replacement Material[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2008,28(5):604-607. | |
| Authors: | Zhixing Zhang Jing Mao Xiangli Feng Jianzhong Xiao Jinjun Qiu |
| Institution: | 1. Department of Orthodontics, Tongji Hospital, Wuhan 430022, China 2. Department of Orthodontics, Tongji Hospital, Wuhan 430022, China;Department of Nursmg, Tong, It Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 3. College of Materials Science and Engineering, Wuhan 430079, China 4. College of Chemistry, Huazhong University of Science and Technology, Wuhan 430079, China |
| Abstract: | The aim of this study was to investigate the in vitro cytotoxicity of polyphosphoester polymer used as a novel injectable alveolar bone substitutes for controlled delivery of tetracycline. Cell culture medium was exposed to the polymer (0.01-10 mg/mL) for 24 h. The L-929 mouse fibroblasts were then exposed to the treated cell culture medium for 24 h. Finally, cell viability and growth were assessed by using MTT assay and Alamar Blue assay. No significant cytotoxicity of the polyphosphoester against L-929 mouse fibroblasts was observed at a concentration up to 10 mg/mL (P>0.05). The two evaluation methods showed no significant differences (P>0.05). This study suggests that polyphosphoester does not demonstrate any significant toxic effects to cells in vitro and has the potential to be used both as a medical device and as scaffolds in tissue engineering applications. |
| Keywords: | polyphosphoester cytotoxicity MTT assay Alamar Blue test |
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