高效液相色谱法测定养阴降糖胶囊中葛根素的含量

目的：建立用高效液相法（HPLC）测定养阴胶囊中葛根素的含量。方法：采用HPLC法测定,色谱柱为AgilentXDBC18（4.6mm×250mm,5μm）,流动相采用甲醇∶磷酸盐溶液=20∶80,流速为1.0ml/min,检查波长250nm,柱温为室温,进样量为10μl。结果：葛根素在0.100～3.100μg范围内线性关系良好（r=0.9996）,平均回收率为98.46%,RSD为0.80%（n=5）。结论：该测定方法简单、可靠、专属性强,可以作为养阴降糖胶囊中葛根素含量的测定方法,从而有效地控制该制剂的含量。

Determination of puerarin in Yangyin Jiangtang Capsule by HPLC

Objective： To determine the contents of the puerarin in Yangyin Jiangtang Capsule by HPLC. Methods： HPLC was used with a column of Agilent XDB C18 （4.6 mm×250 mm, 5 μm） and a mobile phase of methanol-phosphate buffer （20：80）, the flow rate was 1.0 ml/min, the detection wavelength was 250 nm and the injection volume was 10 μl. Results： Puerrarin in the range of 0.100～3.100 μg good linear relationship （r=0.999 6）, the average recovery was 98.46%, RSD was 0.80%（n=5）. Conclusion： The method is simple, reliable, specific, Yin Capsule as the determination of puerarin in order to effectively control the content of the preparation.