| 负载hTERT调控相关miR-138复制缺陷型腺病毒的包装及鉴定 |
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| 引用本文： | 熊晓峰,陈陵,范亚川,邹利全,张方征,王洪斌,游斌,刘志鹏. 负载hTERT调控相关miR-138复制缺陷型腺病毒的包装及鉴定[J]. 重庆医学, 2011, 40(9): 896-898. DOI: 10.3969/j.issn.1671-8348.2011.09.026 |
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| 作者姓名： | 熊晓峰 陈陵 范亚川 邹利全 张方征 王洪斌 游斌 刘志鹏 |
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| 作者单位： | 解放军第三二四医院消化内科,重庆,400020;解放军第三二四医院消化内科,重庆,400020;解放军第三二四医院消化内科,重庆,400020;解放军第三二四医院消化内科,重庆,400020;解放军第三二四医院消化内科,重庆,400020;解放军第三二四医院消化内科,重庆,400020;解放军第三二四医院消化内科,重庆,400020;解放军第三二四医院消化内科,重庆,400020 |
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| 基金项目： | 重庆市自然科学基金资助项目 |
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| 摘 要： | 目的 包装负载人端粒酶逆转录酶(hTERT)调控相关miR-138前体全长cDNA(pre-miR-138)的复制缺陷型腺病毒载体(Ad-miR138).方法 pre-miR-138全基因合成后插入腺病毒穿梭质粒(pDC315-miR138),与腺病毒包装质粒pBHGloxDeltaE1、3Cre共转染人胚肾293细胞...
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| 关 键 词： | RNA指导的DNA聚合酶 微RNAs 人胚肾293细胞 复制缺陷型腺病毒载体 |
Package and identification of replication-deficient adenovirus carrying hTERT-regulated miR-138 |
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| Xiong Xiaofeng,Chen Ling,Fang Yachuan,Zhou Liquan,Zhang Fangzheng,Wang Hongbing,You Bing,Liu Zhipeng. Package and identification of replication-deficient adenovirus carrying hTERT-regulated miR-138[J]. Chongqing Medical Journal, 2011, 40(9): 896-898. DOI: 10.3969/j.issn.1671-8348.2011.09.026 |
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| Authors: | Xiong Xiaofeng Chen Ling Fang Yachuan Zhou Liquan Zhang Fangzheng Wang Hongbing You Bing Liu Zhipeng |
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| Affiliation: | Xiong Xiaofeng,Chen Ling,Fang Yachuan,Zhou Liquan,Zhang Fangzheng,Wang Hongbing,You Bing,Liu Zhipeng(Department of Gastroenterology,324 Hospital of PLA,Chongqing 400020,China) |
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| Abstract: | Objective To construct a replication-deficient recombinant adenovirus carrying hTERT-regulated miR-138.Methods The pre-miR-138 sequence was inserted into downstream of CMV promoter of the adenoviral shuttle plasmid pDC315 in sense direction,and the recombinant plasmid pDC315-miR138 was transfected into HEK293 cell together with plasmid pBHGlox(deltaE1,3) containing adenoviral genome,then the replication-deficient recombinant adenovirus expression vector of pre-miR-138(Ad-miR138) was obtained,and identified ... |
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| Keywords: | RNA-directed DNA polymerase microRNAs HEK293 replication-deficient adenoviral vector |
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