| 石墨炉原子吸收光谱分析法测定大鼠前列腺组织中的镉含量 |
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| 引用本文： | 曹显庆,逯岭松,谢国明. 石墨炉原子吸收光谱分析法测定大鼠前列腺组织中的镉含量[J]. 重庆医学, 2011, 40(2). DOI: 10.3969/j.issn.1671-8348.2011.02.007 |
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| 作者姓名： | 曹显庆 逯岭松 谢国明 |
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| 作者单位： | 曹显庆,Cao Xianqing(重庆医科大学公共卫生学院,400016);逯岭松,谢国明,Lu Lingsong,Xie Guoming(重庆医科大学医学检验系,400016) |
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| 摘 要： | 目的 寻找可以消除大鼠前列腺组织中对镉检测产生干扰的基体改进剂,建立一种采用石墨炉原子吸收分光光度计准确测定大鼠前列腺组织中镉含量的新方法 .方法 取大鼠前列腺组织样本,用基体改进剂(1.0%HNO3+0.5%NH4H2PO4)进行稀释.使用Z-5000石墨炉原子吸收光谱仪全自动进样方法 进行测定.结果 在最适反应条件下,检测大鼠前列腺组织样本相对标准偏差(RSD)为0.12%～4.62%,回收率为95.8%～104.3%.结论 石墨炉原子吸收光谱分析法是一种理想的检测大鼠前列腺组织中镉含量的方法 ,具有良好的准确度和精密度.
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| 关 键 词： | 镉 分光光度法,原子 基体改进剂 大鼠 前列腺 |
Determination of cadmium in prostate of the rats by graphite furnace atomic absorption spectrophotometry |
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| Cao Xianqing,Lu Lingsong,Xie Guoming. Determination of cadmium in prostate of the rats by graphite furnace atomic absorption spectrophotometry[J]. Chongqing Medical Journal, 2011, 40(2). DOI: 10.3969/j.issn.1671-8348.2011.02.007 |
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| Authors: | Cao Xianqing Lu Lingsong Xie Guoming |
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| Affiliation: | Cao Xianqing1,Lu Lingsong2,Xie Guoming2(1.College of Public Health,2.Department of Laboratory Medicine,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | Objective To develop an accurate method for determination of cadmium in prostate of the rats by the graphite furnace atomic absorption spectrometry(GFAAS) using suitable matrix modifer to eliminate the matrix interference in prostate of the rats.Methods After the sample was diluted by 1.0%HNO3+0.5%NH4H2PO4,the cadmium in prostate of the rats was determined by GFAAS.Results Under the optimum experimental conditions,the relative standard deviation of the method was 0.12 % to 4.62%,and recovery was 95.8% to 10... |
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| Keywords: | cadmium spectrophotometry atomic matrix modifier rats prostate |
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