青蒿琥酯对佐剂性关节炎大鼠血IL-17、MMP-3及MMP-9的影响

目的拟验证不同剂量青蒿琥酯对大鼠模型血白细胞介素-17(IL-17)、基质金属蛋白酶-3(MMP-3)及基质金属蛋白酶-9(MMP-9)的影响。方法取雄性Wistar大鼠80只建立佐剂性关节炎大鼠模型,第6天选出右后足和两前足关节炎指数之和大于或等于6的60只大鼠,随机分为模型对照组,2.5、5、10、20、40 mg/(kg.d)青蒿琥酯治疗组,每组10只;另取10只正常Wistar大鼠作为健康对照组。应用ELISA检测各组动物灌服不同剂量的青蒿琥酯后血IL-17、MMP-3及MMP-9的浓度。结果与模型对照组相比,各治疗组大鼠血IL-17、MMP-3及MMP-9的浓度均下降(P<0.01),且下降的幅度与青蒿琥酯的浓度呈正相关(IL-17:r=0.56,P=0.003;MMP-3:r=0.54,P=0.003;MMP-9:r=0.58,P=0.003)。而组间对比,在青蒿琥酯浓度达到20 mg/(kg.d)时抑制作用达到高峰。结论青蒿琥酯能下调佐剂性关节炎大鼠血IL-17、MMP-3及MMP-9的浓度,且下降的幅度与青蒿琥酯的浓度呈正相关。

Influence of artesunate on level of interleukin-17,matrix metalloproteinases-3 and matrix metalloproteinases-9 in rat of adjuvant arthritis

Objective To explore the effect of different doses of artesunate on the level of interleukin-17 and matrix metalloproteinases-3(MMP-3) and matrix metalloproteinases-9(MMP-9) in rat of adjuvant arthritis.Methods 80 clean grade male Wistar rats were selected to establish model adjuvant arthritis.On 6 d after modeling,60 rats with the sum of arthritis index of right metapedes and two propodium ≥6 were selected,and randomly divided into 6 groups(n=10).Model control group was infused with normal saline[10 mL/(kg·day)]for 7 d;the other groups,artesunate groups,were infused with different doses of artesunate[(2.5,5,10,20,40 mg/(kg·day)] and normal saline(2-4 mL)for 7 d.10 normal Wistar rats were selected as controls.On 8 d after treatment,enzyme-linked immunosorbent assay(ELISA)was used to detect serum level of IL-17,MMP-3 and MMP-9.Results Compared with model rats,the serum level of IL-17 and MMP-3 and MMP-9 in artesunate groups were significantly decreased in a dose-depedent manner(P< 0.01).The group[20mg/(kg·day)] was the top in artesunate groups.Conclusion Artesunate can down-regulate the serum level of IL-17,MMP-3 and MMP-9 of adjuvant arthritis in a dose-depentment manner.