IL-10对人早孕蜕膜基质细胞的细胞因子信号抑制因子(SOCSs)基因表达的调控作用

目的:研究细胞因子信号抑制因子SOCS1、SOCS2、SOCS3基因在正常人早孕蜕膜基质细胞的表达及其意义。方法:体外分离培养人早孕蜕膜基质细胞,采用逆转录聚合酶链反应(RT-PCR)及Western blotting法检测SOCS表达,酶联免疫吸附剂测定(ELISA)法检测细胞IL-10、IFN-γ的分泌。结果:无血清培养蜕膜基质细胞未见SOCS1表达,但表达SOCS2、SOCS3;细胞培养14h所分泌的IL-10高于细胞培养2h时的水平(P<0.05),IFN-γ分泌则无明显变化(P>0.05);IL-10(0.1ng/ml)作用蜕膜基质细胞14h内,不同程度上调SOCS2、SOCS3表达。结论:人早孕蜕膜基质细胞自分泌、旁分泌的IL-10可能诱导细胞SOCS2、SOCS3的表达。

Regulatory Expression of Cytokine Signaling (SOCS) in Human Decidual Stromal Cells by IL-10 during First-trimester

Objective: To explore the expression and significance of suppressors of cytokine signaling (SOCS)1, SOCS2, SOCS3 in human decidual stromal cells (DSCs) during first-trimester. Methods: The human DSCs were isolated and cultured in vitro for 48 h, and were serum-starved overnight, then treated with IL-10 for 14 h. Semi-quantitative RT-PCR and Western blotting were used to detect the SOCS1, SOCS2, SOCS3 mRNA and protein expression. IL-10 and IFN-γ secretion were detected by enzyme linked immunosorbent assay (ELISA). Results: The expression of SOCS1 was not detected in DSCs under serum-starved condition forl4 h, but the low expression of SOCS2 and SOCS3 was founded. The level of IL-10 was increased significantly at 14 h compared with that of 2 h under serum-starved condition (P<0.05), and IFN-γ maintained low level within 14 h compared with the secretion of 2 h, P>0.05. The SOCS2 and SOCS3 mRNA and protein expressions were significantly increased in decidual stromal cells response to IL-10 (0.1 ng/ml) respectively. Conclusion: IL-10 may be the one of cause inducing SOCS2 and SOCS3 expression, which autosecreted and parasecreted by DSC during first-trimester.