慢性高原病患者骨髓Cyt C和Caspase-3表达及意义研究

目的 探讨细胞凋亡在慢性高原病（CMS）发生发展过程中的作用,从而寻求新的诊断和治疗途径。方法 选取CMS患者20例为研究对象,健康人群14例为对照组,采用固相双抗体夹心ELISA方法测定CMS组及对照组骨髓上清液Cyt C和Caspase-3水平。结果 CMS患者骨髓上清液中Cyt C水平（21.43±8.92）ng/ml]水平与对照组（10.36±3.55）ng/ml]比较差异有统计学意义（P〈0.05）;CMS患者骨髓上清液中Caspase-3水平（15.45±4.73）ng/ml]水平与对照组（7.14±1.93）ng/ml]比较差异有统计学意义（P〈0.05）;CMS患者骨髓上清液中Cyt C与Caspase-3水平呈正相关（r=0.706,P〈0.01）;CMS患者骨髓上清液中Cyt C和Caspase-3表达水平均与血红蛋白（Hb）浓度呈正相关（r=0.524,P〈0.01;r=0.577,P〈0.01）,与血氧饱和度（SaO2）呈负相关（r=-0.505,P〈0.05;r=-0.554,P〈0.01）。结论 推测细胞凋亡可能在CMS病理生理过程中发挥着重要作用。

Expression of Cyt C and Caspase-3 of bone marrow supernatant in patients with chronic mountain sickness

Objective To investigate the apoptosis occurred during the development of function in CMS, so as to seek a new means of diagnosis and treatment. Methods The bone marrow supematant levels of Cyt C and Caspase-3 in 20 CMS patients and 14 healthy people were determined by quantitative sandwich enzyme immunoassay. Results The bone marrow supematant levels of Cyt C（21.43±8.92）ng/ml] and Caspase-3（10.36±3.55）ng/ml] in CMS were significantly higher than those in control group （ 15.45±4.73 ）ng/ml] and （7.14±1.93 ）ng/ml] respectively. And there were positive relationship between the levels of Cyt C and 12aspase-3 （r=0.706,P〈0.01） ,and between the levels of Cyt C and Hb （r=0.524,P〈0.01）,and also between the levels of Caspase-3 and Hb （r=0.577,P〈0.01）. There were negative relationship between the levels of Cyt C and SaO2（r=-0.554, P〈0.05） ,and between the levels of Caspase-3 and SaO2 （r=-0.505,P〈0.05）. Conclusion Speculate that apoptosis may play an important rote in the pathophysiological processes in CMS.