低温保存成骨细胞复合生物活性玻璃陶瓷修复兔下颌骨缺损

目的 观察经低温保存的兔骨膜源性成骨细胞(POBs)的生物学特征,及其与生物活性玻璃陶瓷(BGC)体外复合后修复颌骨缺损的能力。方法 将经鉴定的幼兔骨膜源性成骨细胞置入液氮罐中保存,取冻存6个月的细胞做生物学鉴定,并进行体外培养扩增,然后与BGC复合培养,植入兔下颌骨缺损处,对照组为植入单纯BGC组。术后第2、4、8、12周取材,行X线摄片及组织学检查,观察复合材料的成骨能力。结果 复苏的成骨细胞仍具有典型的成熟成骨细胞的生物学特征,与BGC复合植入体内后,能继续生长增殖并形成骨组织,能较快较好地修复骨缺损。结论 利用冻存复苏的成骨细胞进行组织工程学研究是可行的,细胞与材料复合所形成的组织工程化骨,可望在骨组织的修复与重建中得到更加广泛的应用。

The Experimental Study of Cryopreserved Osteoblasts Combined with BGC Repairing Mandibular Defects in Rabbits

Objective The purpose of this study was to observe the biological characteristics of cultured Periosteal-de-rived Osteoblasts(POBs)preserved in liquid nitrogen in vitro,and to preliminarily study the osteogenetic capability of bioactive glass ceramic(BGC)combined with POBs.Methods The cryopreserved cells were cultured in DMEM medium and examined by morphological and histological assay.The POBs growing well in vitro were seeded into the porous BGC materials.A week later,the combined materials were implanted into the bone defects of rabbits' mandibular,the control groups were implanted into the single BGC(no cells).After2,4,8,12weeks of operation,the specimens were respectively excised and examined by X-ray and histological chemistry.Results The cryopreserved POBs grew well in vitro and also had the tipical characteristics of mature osteoblasts.Cultured with BGC materials,the cells could attach,grow and prolifer-ate well on the surface of most endoporous.After4weeks of operation,the transplanted osteoblasts began to form new os-teoid or bone tissue in most pores of implanted BGC,and the bone defects were repaired better and earlier.Conclusion It was practical to use the cryopreserved osteoblasts for further study on bone tissue engineering.It suggested that the"life active"bone would get more application and play a more important role in bone restoration and reconstruction.