模拟失重对大鼠胃黏膜瘦素及其受体表达的影响

目的：观察模拟失重条件下大鼠胃黏膜瘦素及其受体（OB-R）表达的变化,探讨失重对消化系统功能的影响.方法：将22只SD大鼠分为模拟失重组（n=12）和正常对照组（n=10）,采用尾悬吊模拟失重28d,用免疫组化ABC方法,观察大鼠胃黏膜瘦素及其受体的定位与表达,并比较两组动物胃黏膜瘦素及其受体免疫反应阳性细胞密度的差异.结果：瘦素及其受体免疫反应阳性细胞均分布于胃底腺的中下部区域、阳性物质主要定位于主细胞和壁细胞.与正常对照组相比,模拟失重组大鼠胃黏膜瘦素免疫反应阳性细胞密度显著增加（P＜0.01）;而瘦素受体免疫反应阳性细胞密度虽有增加趋势,但无显著差异（P＞0.05）. 结论：28 d的悬吊模拟失重可致大鼠胃黏膜瘦素及其受体表达增加,提示瘦素可能参与失重过程中消化道功能紊乱的调节.

Effect of simulated weightlessness on leptin and leptin receptor expression in rat gastric mucosa

Objective: To investigate the expression of leptin and its receptor(OB-R) in gastric mucosa of rats following 28 day tail-suspension. Methods:Twenty two Sprague-Dawley rats were divided into 28 day tail-suspension group(n=12) and in-phase normal control group(n=10).Localization and expression of leptin and OB-R in rat gastric mucosa was observed by immunohistochemistry,and the density of leptin and OB-R immunoreactive cells in both groups were compared. Results:Leptin and OB-R immunoreactive cells were distributed in the middle and low part of the fundic gastric gland,and the positive staining signals were mainly located in chief as well as parietal cell.Leptin immunoreactive cell density in the 28 day tail-suspension group were significantly increased compared with the in-phase normal control group(P<0.01).While OB-R immunoreactive cell density in the 28 day tail-suspension rats were increased than those of control rats,but the difference was not statistically significant(P>0.05).Conclusion: Ground simulated weightlessness induced by 28 day tail-suspension in rats can lead to the enhanced expression of leptin and OB-R in gastric mucosa.